Three-Dimensional Microfibrous Bundle Structure Fabricated Using an Electric Field-Assisted/Cell Printing Process for Muscle Tissue Regeneration,ACS Biomaterials Science & Engineering

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Three-Dimensional Microfibrous Bundle Structure Fabricated Using an Electric Field-Assisted/Cell Printing Process for Muscle Tissue Regeneration
ACS Biomaterials Science & Engineering ( IF 5.4 ) Pub Date : 2018-01-22 00:00:00 , DOI: 10.1021/acsbiomaterials.7b00983
Miji Yeo ₁ , GeunHyung Kim ₁

Affiliation

In tissue engineering, biomimetic scaffolds are developed to provide cells with a microenvironment that promotes cellular activities. In this study, we present a three-dimensional (3D) fibrous bundle structure fabricated using an electrohydrodynamic process and a cell printing process using myoblast-laden collagen bioink. An anisotropic topographical cue in a 3D structure is an important factor for muscle tissue regeneration, and therefore, the fibrous bundle structure was uniaxially stretched using optimized conditions for fiber alignment. In addition, for stable cell attachment to facilitate the effect of topological cues, the myoblasts were efficiently released from the collagen bioink. We observed that the 3D fibrous bundle structure was an effective in vitro platform that induced cell proliferation and the formation of myotubes. The synergistic combination of the aligned topological cues and high biocompatibility of collagen enhanced the formation of myotubes, which was represented by the relative expression of myogenic genes (Myf5, Myh2, MyoD, and Myogenin). Therefore, we could confirm the feasibility of the 3D fibrous bundle structure for the regeneration of skeletal muscle tissues.

中文翻译：

使用电场辅助/细胞印刷工艺制造的三维微纤维束结构用于肌肉组织再生

在组织工程中，仿生支架被开发为提供具有促进细胞活性的微环境的细胞。在这项研究中，我们介绍了三维（3D）纤维束结构，使用电流体动力学过程和细胞打印过程使用成肌细胞负载胶原蛋白生物墨水制作而成。3D结构中的各向异性地形线索是肌肉组织再生的重要因素，因此，使用用于纤维排列的最佳条件对纤维束结构进行了单轴拉伸。另外，为了稳定的细胞附着以促进拓扑提示的作用，成肌细胞被有效地从胶原生物墨水释放。我们观察到3D纤维束结构是诱导细胞增殖和肌管形成的有效体外平台。对齐的拓扑线索和胶原蛋白的高生物相容性的协同组合增强了肌管的形成，这由成肌基因（Myf5，Myh2，MyoD和Myogenin）的相对表达来表示。因此，我们可以确认3D纤维束结构在骨骼肌组织再生中的可行性。

更新日期：2018-01-22

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