We report on the preparation of electropositive nanofiber membranes by electrospinning with in situ photo-crosslinking and their preliminary evaluation in virus adsorption and removal tests. Poly(vinyl alcohol) (PVA) and polyethyleneimine (PEI) were modified with glycidyl methacrylate, to form an acrylated crosslinked polymer (a-PVA/a-PEI) upon UV exposure during the electrospinning process. The a-PVA/a-PEI nanofibers were electrospun on a non-woven polyester support to form an electropositive (ζ = 7 mV at pH 7.4) and hydrophilic (${\theta }_w\sim {53}^{\text{°}}$) membrane with the mean pore size of 0.48 μm. The microfilter had the specific permeate flux of ∼6.9 · 10⁴ L/(m²·h·bar), comparable with that of commercially available membranes of similar nominal pore sizes. Adsorption of the negatively charged and hydrophilic bacteriophage MS2 ($d\sim 27$ nm) onto the membrane followed Freundlich isotherm and could be classified as favorable with the average adsorption intensity $n^{-1}\sim 0.91$. The 99% retention of MS2 in flow-through virus clearance tests was attributed to adsorption and was likely controlled by the limited detention time within the membrane.

我们报告了静电纺原位光交联制备电正性纳米纤维膜及其在病毒吸附和去除测试中的初步评估。聚乙烯醇（PVA）和聚乙烯亚胺（PEI）用甲基丙烯酸缩水甘油酯改性，在静电纺丝过程中受到紫外线照射后形成丙烯酸酯化的交联聚合物（a-PVA / a-PEI）。将a-PVA / a-PEI纳米纤维电纺在非织造聚酯载体上，以形成正电（在pH 7.4时ζ= 7 mV）和亲水性（${\theta }_w〜{53}^{\text{°}}$膜的平均孔径为0.48μm。该微滤器的比渗透通量约为6.9·10 ⁴  L /（m ² ·h·bar），与具有相似标称孔径的市售膜相当。带负电荷的亲水性噬菌体MS2（$d〜27$ 到Freundlich等温线之后，在膜上的平均吸收强度可被分类为有利的 ${ñ}^{--\mathrm{1个}}〜0.91$。在流通型病毒清除测试中，MS2的99％保留归因于吸附，并且很可能受膜内有限的保留时间控制。