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Design of a Single-Layer Microchannel for Continuous Sheathless Single-Stream Particle Inertial Focusing
Analytical Chemistry ( IF 6.7 ) Pub Date : 2018-01-17 00:00:00 , DOI: 10.1021/acs.analchem.7b03756 Yan Zhang 1 , Jun Zhang 2 , Fei Tang 1 , Weihua Li 3 , Xiaohao Wang 1
Analytical Chemistry ( IF 6.7 ) Pub Date : 2018-01-17 00:00:00 , DOI: 10.1021/acs.analchem.7b03756 Yan Zhang 1 , Jun Zhang 2 , Fei Tang 1 , Weihua Li 3 , Xiaohao Wang 1
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High-throughput, high-precision single-stream focusing of microparticles has a potentially wide range of applications in biochemical analysis and clinical diagnosis. In this work, we develop a sheathless three-dimensional (3D) particle-focusing method in a single-layer microchannel. This novel microchannel consists of periodic high-aspect-ratio curved channels and straight channels. The proposed method takes advantage of both the curved channels, which induce Dean flow to promote particle migration, and straight channels, which suppress the remaining stirring effects of Dean flow to stabilize the achieved particle focusing. The 3D particle focusing is demonstrated experimentally, and the mechanism is analyzed theoretically. The effects of flow rate, particle size, and cycle number on the focusing performance were also investigated. The experimental results demonstrate that polystyrene particles with diameters of 5–20 μm can be focused into a 3D single file within seven channel cycles, with the focusing accuracy up to 98.5% and focusing rate up to 98.97%. The focusing throughput could reach up to ∼105 counts/min. Furthermore, its applicability to biological cells is also demonstrated by 3D focusing of HeLa and melanoma cells and bovine blood cells in the proposed microchannel. The proposed sheathless passive focusing scheme, featuring a simple channel structure, small footprint (9 mm × 1.2 mm), compact layout, and uncomplicated fabrication procedure, holds great promise as an efficient 3D focusing unit for the development of next-generation on-chip flow cytometry.
中文翻译：
连续无鞘单流粒子惯性聚焦的单层微通道设计
微粒的高通量，高精度单流聚焦在生化分析和临床诊断中具有潜在的广泛应用。在这项工作中，我们开发了单层微通道中的无鞘三维（3D）粒子聚焦方法。这种新颖的微通道由周期性的高纵横比弯曲通道和直通道组成。所提出的方法利用了引起迪安流动以促进颗粒迁移的弯曲通道和通过抑制迪安流动的剩余搅拌效果以稳定所获得的颗粒聚焦的直通道。实验证明了3D粒子聚焦，并从理论上分析了其机理。还研究了流速，粒径和循环次数对聚焦性能的影响。实验结果表明，直径为5–20μm的聚苯乙烯颗粒可以在七个通道周期内聚焦到3D单个文件中，聚焦精度高达98.5％，聚焦率高达98.97％。聚焦通量可以达到约105个计数/分钟。此外，通过在建议的微通道中对HeLa和黑色素瘤细胞以及牛血细胞进行3D聚焦，也证明了其对生物细胞的适用性。拟议的无鞘被动聚焦方案具有简单的通道结构，较小的占位面积（9 mm×1.2 mm），紧凑的布局以及简单的制造程序，作为开发下一代片上芯片的高效3D聚焦单元，具有广阔的前景流式细胞仪。
更新日期：2018-01-17
中文翻译：
连续无鞘单流粒子惯性聚焦的单层微通道设计
微粒的高通量，高精度单流聚焦在生化分析和临床诊断中具有潜在的广泛应用。在这项工作中，我们开发了单层微通道中的无鞘三维（3D）粒子聚焦方法。这种新颖的微通道由周期性的高纵横比弯曲通道和直通道组成。所提出的方法利用了引起迪安流动以促进颗粒迁移的弯曲通道和通过抑制迪安流动的剩余搅拌效果以稳定所获得的颗粒聚焦的直通道。实验证明了3D粒子聚焦，并从理论上分析了其机理。还研究了流速，粒径和循环次数对聚焦性能的影响。实验结果表明，直径为5–20μm的聚苯乙烯颗粒可以在七个通道周期内聚焦到3D单个文件中，聚焦精度高达98.5％，聚焦率高达98.97％。聚焦通量可以达到约105个计数/分钟。此外，通过在建议的微通道中对HeLa和黑色素瘤细胞以及牛血细胞进行3D聚焦，也证明了其对生物细胞的适用性。拟议的无鞘被动聚焦方案具有简单的通道结构，较小的占位面积（9 mm×1.2 mm），紧凑的布局以及简单的制造程序，作为开发下一代片上芯片的高效3D聚焦单元，具有广阔的前景流式细胞仪。
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