Altering 2‐Hydroxybiphenyl 3‐Monooxygenase Regioselectivity by Protein Engineering for the Production of a New Antioxidant,ChemBioChem

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Altering 2‐Hydroxybiphenyl 3‐Monooxygenase Regioselectivity by Protein Engineering for the Production of a New Antioxidant
ChemBioChem ( IF 2.6 ) Pub Date : 2018-02-08 , DOI: 10.1002/cbic.201700648
Almog Bregman-Cohen ₁ , Batel Deri ₁ , Shiran Maimon ₁ , Yael Pazy ₂ , Ayelet Fishman ₁

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New regiospecificity→new antioxidant: One single mutation, M321A, changed 2‐hydroxybiphenyl 3‐monooxygenase (HbpA) regioselectivity from oxidizing 2‐substituted phenols to 3‐substituted phenols. The crystal structure provided an explanation for the new activity. The antioxidant produced, 3,4‐dihydroxybiphenyl, had similar ferric reducing capacity to the well‐studied piceatannol.

中文翻译：

通过蛋白质工程改变2-羟基联苯3-单加氧酶的区域选择性以生产新的抗氧化剂

新的区域特异性→新的抗氧化剂：一个单一突变M321A改变了2-羟基联苯3-单加氧酶（HbpA）的区域选择性，从将2-取代的苯酚氧化为3-取代的酚。晶体结构为新活性提供了解释。产生的抗氧化剂3,4-二羟基联苯与经过充分研究的皮卡季诺醇具有类似的三价铁还原能力。

更新日期：2018-02-08

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