We show that mannan-based conjugates possess exceptional features for multimodal imaging because of their biocompatibility, biodegradability and self-targeting properties. Two new mannan conjugates, containing a gadolinium complex and a fluorescent probe, one based only on polysaccharide and the other one comprising polysaccharide with poly(2-methyl-2-oxazoline) grafts, were prepared and simultaneously visualized in vitro and in vivo by magnetic resonance and fluorescence imaging. The synthesis of these mannan-based complexes was based on alkylation with allyl bromide or grafting with poly(2-methyl-2-oxazoline) chains, followed by a thiol–ene click reaction with cysteamine to introduce primary amino groups into their structure. Finally, the obtained conjugates were functionalized with contrast labels using the corresponding N-hydroxysuccinimide esters. When used to detect lymph nodes, the polymers showed better imaging properties than a commercially available contrast agent.

中文翻译：

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基于甘露聚糖的结合物可作为淋巴结的多峰成像平台†

我们显示，基于甘露聚糖的结合物具有生物相容性，生物降解性和自我靶向特性，因此具有用于多峰成像的出色功能。两个新的甘露聚糖结合物，含有钆络合物和荧光探针，一个仅基于多糖，而另一个包括与聚（2-甲基-2-恶唑啉）移植物多糖，制备和同时可视化在体外和体内通过磁共振和荧光成像。这些基于甘露聚糖的配合物的合成基于烯丙基溴的烷基化或聚（2-甲基-2-恶唑啉）链的接枝，然后与半胱胺的硫醇-烯点击反应将伯氨基引入其结构。最后，使用相应的N-羟基琥珀酰亚胺酯，用对比标记将获得的缀合物官能化。当用于检测淋巴结时，该聚合物显示出比市售造影剂更好的成像性能。