A far-red-emissive AIE active fluorescent probe with large stokes shift for detection of inflammatory bowel disease in vivo†,Journal of Materials Chemistry B

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A far-red-emissive AIE active fluorescent probe with large stokes shift for detection of inflammatory bowel disease in vivo†
Journal of Materials Chemistry B ( IF 6.1 ) Pub Date : 2018-01-03 00:00:00 , DOI: 10.1039/c7tb03168h
Wanfu Xu _{1,

2,

3,

4,

5} , Liying He _{1,

2,

3,

4,

5} , Qi Xia _{4,

5,

6,

7} , Chunhong Jia _{4,

5,

8,

9} , Lanlan Geng _{1,

2,

3,

4,

5} , Min Yang _{1,

2,

3,

4,

5} , Zhaohui Xu _{1,

2,

3,

4,

5} , Peiyu Chen _{1,

2,

3,

4,

5} , Yang Cheng _{1,

2,

3,

4,

5} , Junhong Zhao _{1,

2,

3,

4,

5} , Hongli Wang _{1,

2,

3,

4,

5} , Huan Chen _{1,

2,

3,

4,

5} , Yuhua Zhang _{5,

8,

10,

11} , Sitang Gong _{1,

2,

3,

4,

5} , Ruiyuan Liu _{4,

5,

6,

7}

Affiliation

Inflammatory bowel disease (IBD) is a group of chronic remittent or progressive inflammatory gastrointestinal tract diseases, accompanying impaired barrier function. Effective real-time monitoring of the IBD progression in time is critical to the success of bowel therapy and the improvement of patient outcomes. However, there is relatively little information regarding the detection of the intestinal barrier. In this study, we have judiciously designed and synthesized a novel D–π–A based far-red-emissive AIE active fluorescent Probe (PTZB-FR). PTZB-FR exhibits properties suitable for in vivo imaging, such as FR emission, good photostability, large Stokes shift (∼184 nm), and low cytotoxicity. Moreover, confocal fluorescence microscopy and flow cytometry assay reveal the effective internalization of PTZB-FR. Most importantly, imaging living mice after oral administration of PTZB-FR confirms that fluorescence in the intestinal lumen diminishes in Dextran sulfate sodium (DSS)-induced colitis, which is attributed to disruption of intestinal barrier integrity caused by a DSS-induced colitis model, highlighting PTZB-FR as a promising reagent in the detection of intestinal barrier function during clinical examination.

中文翻译：

远行程红色AIE主动荧光探针，用于检测体内炎症性肠病†

炎性肠病（IBD）是一组慢性缓解或进行性炎性胃肠道疾病，伴有屏障功能受损。及时有效地实时监测IBD进展对肠治疗的成功和患者预后的改善至关重要。但是，关于肠壁屏障的检测的信息相对较少。在这项研究中，我们明智地设计和合成了一种基于D–π–A的新型远红外发射AIE有源荧光探针（PTZB-FR）。PTZB-FR具有适合体内的特性成像，例如FR发射，良好的光稳定性，大的斯托克斯位移（〜184 nm）和低的细胞毒性。此外，共聚焦荧光显微镜和流式细胞术测定揭示了PTZB-FR的有效内在化。最重要的是，口服PTZB-FR后对活体小鼠进行成像证实，肠腔中的荧光在硫酸葡聚糖硫酸钠（DSS）引起的结肠炎中减少，这归因于由DSS诱导的结肠炎模型引起的肠屏障完整性破坏，强调PTZB-FR是在临床检查过程中检测肠屏障功能的有前途的试剂。

更新日期：2018-01-03

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