Synergetic topography and chemistry cues guiding osteogenic differentiation in bone marrow stromal cells through ERK1/2 and p38 MAPK signaling pathway,Biomaterials Science

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Synergetic topography and chemistry cues guiding osteogenic differentiation in bone marrow stromal cells through ERK1/2 and p38 MAPK signaling pathway
Biomaterials Science ( IF 5.8 ) Pub Date : 2018-01-03 00:00:00 , DOI: 10.1039/c7bm01044c
Xinran Zhang _{1,

2,

3,

4,

5} , Haotian Li _{5,

6,

7,

8,

9} , Chucheng Lin _{5,

10,

11,

12} , Congqin Ning _{5,

10,

11,

12} , Kaili Lin _{1,

2,

3,

4,

5}

Affiliation

Both the topographic surface and chemical composition modification can enhance rapid osteogenic differentiation and bone formation. Till now, the synergetic effects of topography and chemistry cues guiding biological responses have been rarely reported. Herein, the ordered micro-patterned topography and classically essential trace element of strontium (Sr) ion doping were selected to imitate topography and chemistry cues, respectively. The ordered micro-patterned topography on Sr ion-doped bioceramics was successfully duplicated using the nylon sieve as the template. Biological response results revealed that the micro-patterned topography design or Sr doping could promote cell attachment, ALP activity, and osteogenic differentiation of bone marrow-derived mesenchymal stem cells (BMSCs). Most importantly, the samples both with micro-patterned topography and Sr doping showed the highest promotion effects, and could synergistically activate the ERK1/2 and p38 MAPK signaling pathways. The results suggested that the grafts with both specific topography and chemistry cues have synergetic effects on osteogenic activity of BMSCs and provide an effective approach to design functional bone grafts and cell culture substrates.

中文翻译：

通过ERK1 / 2和p38 MAPK信号传导途径指导骨髓基质细胞成骨分化的协同地形学和化学线索

地形表面和化学成分的修饰都可以促进快速的成骨分化和骨形成。迄今为止，很少有地形学和化学指导生物反应的协同作用的报道。在此，选择有序的微图案形貌和锶（Sr）离子掺杂的经典必需微量元素分别模拟形貌和化学线索。使用尼龙筛作为模板，成功地复制了Sr离子掺杂生物陶瓷上的有序微图案形貌。生物学反应结果表明，微模式的地形设计或Sr掺杂可以促进骨髓间充质干细胞（BMSCs）的细胞附着，ALP活性和成骨分化。最重要的是，具有微图案形貌和Sr掺杂的样品显示出最高的促进作用，并且可以协同激活ERK1 / 2和p38 MAPK信号通路。结果表明，具有特定形貌和化学提示的移植物对骨髓间充质干细胞的成骨活性具有协同作用，为设计功能性骨移植物和细胞培养基质提供了有效的途径。

更新日期：2018-01-03

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