A rapid, simple, and sensitive fluorescent immunochromatographic strip test (ICST) based on quantum dots (QDs) has been developed to detect 1-aminohydantoin (AHD), a major metabolite of nitrofurantoin in animal tissues. To achieve this, QD-labeled antibody conjugates, which consist of CdSe/ZnS QDs and monoclonal antibodies, were prepared by an activated ester method. Under optimal conditions, with the nitrophenyl derivative of AHD as the target, the ICST had a linear range from 0.1 to 100 ng/mL, with a correlation coefficient of 0.9656 and a 50% inhibitory concentration of 4.51 ng/mL. The limit of detection was 0.14 ng/g, which was below the minimum required performance limit of 1 μg/kg for AHD established by the European Commission. The recoveries for AHD ranged from 81.5% to 108.2%, with coefficients of variation below 13%, based on intraday and interday analysis. Furthermore, for AHD in real samples, the ICST showed high reliability and high correlation with liquid chromatography–tandem mass spectrometry (correlation coefficient of 0.9916). To the best of our knowledge, this is the first report of a novel and sensitive method based on a fluorescent ICST to detect AHD below the minimum required performance limit. The ICST demonstrated high reliability, and could be ideally suited for rapid, simple, and on-site screening of AHD contamination in animal tissues.

中文翻译：

---

使用量子点抗体探针的荧光免疫层析试纸条，用于快速定量检测可食用动物组织中的1-氨基乙内酰脲

已经开发了一种基于量子点（QD）的快速，简单且灵敏的荧光免疫层析试纸（ICST），用于检测动物组织中硝基呋喃妥因的主要代谢物1-氨基乙内酰脲（AHD）。为此，通过活化酯法制备了由CdSe / ZnS QD和单克隆抗体组成的QD标记抗体偶联物。在最佳条件下，以AHD的硝基苯基衍生物为目标，ICST的线性范围为0.1至100 ng / mL，相关系数为0.9656，50％抑制浓度为4.51 ng / mL。检出限为0.14 ng / g，低于欧盟委员会制定的AHD的最低要求性能限1μg/ kg。AHD的回收率从81.5％到108.2％，变异系数低于13％，根据日内和日间分析。此外，对于真实样品中的AHD，ICST与液相色谱-串联质谱法具有很高的可靠性和高度相关性（相关系数为0.9916）。据我们所知，这是基于荧光ICST的新颖灵敏方法的首次报道，该方法可检测低于最低性能要求的AHD。ICST具有很高的可靠性，非常适合快速，简单和现场筛查动物组织中AHD污染。这是基于荧光ICST的新颖而灵敏的方法的首次报道，该方法可检测低于最低性能要求的AHD。ICST具有很高的可靠性，非常适合快速，简单和现场筛查动物组织中AHD污染。这是基于荧光ICST的新颖而灵敏的方法的首次报道，该方法可检测低于最低性能要求的AHD。ICST具有很高的可靠性，非常适合快速，简单和现场筛查动物组织中AHD污染。