Thiol–ene radical coupling is increasingly used for the biofunctionalization of biomaterials. Thiol–ene chemistry presents interesting features that are particularly attractive for platforms requiring specific reactions with peptides or proteins and the patterning of cells, such as reactivity in physiological conditions and photoactivation. In this work, we synthesized alkene-functionalized (allyl and norbornene residues) antifouling polymer brushes (based on poly(oligoethylene glycol methacrylate)) and studied thiol–ene coupling with a series of thiols including cell adhesive peptides RGD and REDV. The adhesion of umbilical vein endothelial cells (HUVECs) to these interfaces was studied and highlighted the absence of specific integrin engagement to REDV, in contrast to the high level of cell spreading observed on RGD-functionalized polymer brushes. This revealed that α₄β₁ integrins (binding to REDV sequences) are not sufficient on their own to sustain HUVEC spreading, in contrast to α_vβ₃ and α₅β₁ integrins. In addition, we photopatterned peptides at the surface of poly(oligoethylene glycol methacrylate) (POEGMA) brushes and characterized the quality of the resulting arrays by epifluorescence microscopy and atomic force microscopy (AFM). This allowed the formation of cell patterns and demonstrated the potential of thiol–ene based photopatterning for the design of cell microarrays.

中文翻译：

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通过硫醇-烯偶联的生物功能化图案化聚合物刷，用于控制细胞粘附和细胞阵列的形成

硫醇-烯基自由基偶联越来越多地用于生物材料的生物功能化。巯基-烯化学具有有趣的特征，对于需要与肽或蛋白质发生特定反应以及细胞形成图案的平台（例如在生理条件下的反应性和光激活），这些特征特别有吸引力。在这项工作中，我们合成了烯烃官能化的（烯丙基和降冰片烯残基）防污聚合物刷（基于聚（甲基丙烯酸低聚乙二醇酯）），并研究了硫醇-烯与一系列硫醇的偶联，包括细胞粘附肽RGD和REDV。研究了脐静脉内皮细胞（HUVEC）对这些界面的粘附性，并强调了缺乏特定整合素与REDV的结合，与在RGD官能化的聚合物刷上观察到的高水平细胞扩散相反。这表明α₄ β ₁整合素（结合REDV序列）不足以靠自己来维持HUVEC扩频，而相比之下，α _v β ₃和α _5点β ₁整联。此外，我们在聚（甲基丙烯酸低聚乙二醇酯）（POEGMA）刷子的表面上对肽进行了光图案化，并通过落射荧光显微镜和原子力显微镜（AFM）表征了所得阵列的质量。这样就可以形成细胞模式，并证明了基于硫醇-烯的光图案化技术在细胞微阵列设计中的潜力。