It has been known that metabolism of benzene is necessary for its toxicity. The purpose of our study is to investigate the effect of diallyl trisulfide (DATS) on attenuating cytopenia in peripheral blood introduced by benzene through regulating benzene metabolism in rats. We established benzene poisoning model with benzene (1.3 g/kg), while the DATS treatment groups were treated with DATS plus benzene (15 or 30 mg/kg) for 28 days, respectively. The results of blood parameters and concentration of metabolites of benzene (t, t-MA and SPMA) determination in urine showed that DATS could effectively attenuate the cytopenia induced by benzene through regulating benzene metabolism. Western blot and chemical method were used to detect the activities and protein expression levels of enzymes CYP2E1 and GSTT1 in liver and enzymes MPO and NQO1 in bone marrow were tested. The results suggested that the inhibition of bioactivation in liver and bone marrow catalyzed by CYP2E1 and MPO and the activation of detoxification catalyzed by GSTT1 and NQO1 might be the critical mechanism, through which DATS modulated benzene metabolism to prevent benzene-induced cytopenia.

已知苯的代谢对其毒性是必需的。我们的研究目的是研究三烯丙基三硫化物（DATS）通过调节大鼠体内苯代谢，减轻苯引起的外周血细胞减少症的作用。我们建立了苯（1.3 g / kg）的苯中毒模型，而DATS治疗组分别接受了DATS加苯（15或30 mg / kg）治疗28天。尿液中血液参数和苯代谢物浓度（t，t-MA和SPMA）的测定结果表明，DATS可以通过调节苯的代谢来有效减轻苯诱发的血细胞减少。用蛋白质印迹法和化学法检测肝脏中CYP2E1和GSTT1酶的活性和蛋白表达水平，并检测骨髓中MPO和NQO1酶的表达。结果表明，CYP2E1和MPO催化抑制肝脏和骨髓的生物活化以及GSTT1和NQO1催化解毒的活化可能是关键机制，DATS可以通过该机制调节苯代谢以防止苯诱导的血细胞减少。