Abstract The production of new multi-responsive poly(N-isopropylacrylamide-allyl acetic acid) [P(NIPAM-AAA)] copolymer microgel by free radical emulsion polymerization is reported. Inside this copolymer microgel CuO nanoparticles were generated by in situ reduction of copper nitrate followed by the air oxidation process, their fabrication is confirmed by powdered X-ray diffraction analysis and the average size of CuO NPs was found to be 24 nm having monoclinic shapes. By using dynamic laser light scattering the swelling & de-swelling behaviour of the pure microgel was examined at different temperature and pH values. The copolymer microgel becomes unstable at low pH as well as at high temperature values respectively. UV–visible spectra show a red shift in surface plasmon resonance λSPR of CuO nanoparticles. The catalytic property of hybrid microgel was inspected by observing the reduction of 4-nitrophenol into 4-aminophenol in the presence of excess NaBH4 with various concentrations of catalyst at room temperature. The hybrid microgels also have good anti-bacterial activity against both Gram-positive (C. albicans) and Gram-negative (E. coli) bacteria.

中文翻译：

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在 P(NIPAM-co-AAA) 微凝胶中原位合成 CuO 纳米颗粒、结构表征、催化和生物应用

摘要 报道了通过自由基乳液聚合制备新型多响应聚（N-异丙基丙烯酰胺-烯丙基乙酸）[P(NIPAM-AAA)]共聚物微凝胶。在该共聚物微凝胶中，CuO 纳米颗粒是通过硝酸铜的原位还原和空气氧化过程产生的，它们的制造通过粉末 X 射线衍射分析得到证实，并且发现 CuO NPs 的平均尺寸为 24 nm，具有单斜晶形。通过使用动态激光散射，研究了纯微凝胶在不同温度和 pH 值下的溶胀和消溶胀行为。共聚物微凝胶分别在低 pH 值和高温值下变得不稳定。UV-可见光谱显示CuO纳米颗粒的表面等离子体共振λSPR发生红移。混合微凝胶的催化性能通过在室温下观察在过量 NaBH4 和不同浓度催化剂存在下 4-硝基苯酚还原为 4-氨基苯酚的情况来检测。混合微凝胶还对革兰氏阳性（白色念珠菌）和革兰氏阴性（大肠杆菌）细菌具有良好的抗菌活性。