Structural characterization of two endopolysaccharides from Phellinus sp. and their immunologic effects by intragastric administration in a healthy mammalian model†,Food & Function

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Structural characterization of two endopolysaccharides from Phellinus sp. and their immunologic effects by intragastric administration in a healthy mammalian model†
Food & Function ( IF 5.1 ) Pub Date : 2018-01-03 00:00:00 , DOI: 10.1039/c7fo01161j
Xiao-kui Ma _{1,

2,

3,

4,

5} , Yao Ma _{1,

2,

3,

4,

5} , Eric Charles Peterson _{6,

7,

8,

9} , Wen ying Guo _{1,

2,

3,

4,

5} , Zhong Yu Li _{10,

11,

12,

13} , Yu Li _{13,

14,

15,

16}

Affiliation

Key Laboratory of Medicinal Resources and Natural Pharmaceutical Chemistry
Ministry of Education
National Engineering Laboratory for Resource Developing of Endangered Chinese Crude Drugs in Northwest of China
College of Life Science
Shaanxi Normal University
Dept. of Biochemical Engineering
Universidad ICESI
Cali
Colombia
Xi'an Centre of Geological Survey
China Geological Survey
Xi'an 710054
People's Republic of China
School of Science
Northwestern Polytechnical University
Xi'an

Two purified endopolysaccharides derived from cultured Phellinus sp., individually named SHIP-1 and 2, were structurally characterized, along with an evaluation of their in vivo influential immunomodulatory activity in a healthy mammalian model. The structure of SHIP-1 was mainly composed of →4)-α-D-Fucp-(1→, →3,6)-α-D-Araf-(1→ and →2,4)-β-D-Galp-(→, with four residuals of α-D-Manp-(1→ and one α-D-Glcp-(1→ as sidegroups, while the planar structure and the heteronuclear multiple-bond correlation of SHIP-2 were not able to be analyzed. Biochemical analysis in the healthy mice model demonstrated that SHIP-1 increased the concentrations of the detected cytokines in a dosage-dependent manner but not in a time-dependent way. SHIP-2 exerted a positive effect in a dose-dependent manner over time for interferon gamma (IFN-γ) and interleukin (IL)-2 cytokine production at elevated dosages of 200, or 350 mg kg⁻¹ d⁻¹, while IFN-alpha(α) and IL-4 production was observed only in a dosage-dependent manner even at high dosages. Thus, SHIP-1 and 2 significantly improved the immune response through the intragastric administration of the tested high dosages by increasing the production of cytokines in the healthy mice, and these polysaccharides could possibly be used as an immunopotentiator in health foods or dietary supplements.

中文翻译：

桑黄属两种内多糖的结构表征。在健康的哺乳动物模型中通过胃内给药及其免疫学作用†

从培养的桑黄属物种衍生的两种纯化的内多糖，分别命名为SHIP-1和2，在结构上进行了表征，并对它们在健康的哺乳动物模型中的体内影响免疫调节活性进行了评估。SHIP-1的结构主要由→4）-α-的d -Fucp-（1→，→3,6）-α- d -Araf-（1→和→2,4）-β- d - Galp-（→，有四个残差α- D -Manp-（1→和一个α- D-Glcp-（1→为侧基，但无法分析SHIP-2的平面结构和异核多键相关性。在健康小鼠模型中的生化分析表明，SHIP-1增加了检测到的细胞因子的浓度SHIP-2随着时间的推移以剂量依赖性的方式对剂量升高的干扰素γ（IFN-γ）和白介素（IL）-2细胞因子的产生产生积极的影响。 200或350 mg kg ^-1 d ^-1，而IFN-α（α）和IL-4的产生仅以剂量依赖的方式观察到，即使在高剂量下也是如此。因此，SHIP-1和2通过增加健康小鼠体内细胞因子的产生，通过在胃内施用经测试的高剂量来显着改善免疫应答，并且这些多糖可能在健康食品或膳食补充剂中用作免疫增强剂。

更新日期：2018-01-03

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