In the current study, a set of biodegradable soybean polysaccharide (SSPS) nanocomposites containing different ratios of TiO₂ nanoparticles was characterized as new packaging system. X-ray diffraction (XRD) measurement showed that the crystalline structure of the TiO₂ nanoparticles remained intact in the polysaccharide matrix and the surface of nanocomposites containing 1 to 3% TiO₂ was observed morphologically uniform under scanning electron microscopy (SEM). Dynamic mechanical thermal analysis revealed that the magnitude of storage modulus was 3.62-fold higher in SPSS/TiO₂ nanocomposites containing 7 wt. % of TiO₂ than control SSPS indicating improvement in the physical properties of the film supposed to be utilized for food packaging. With respect to the concern over the safety of these nanocomposites, inductively coupled plasma-optical emission spectroscopy (ICP-OES) showed that no TiO₂ was detected in bread samples covered by SSPS/TiO₂ film and stored for 6 months. Similarly, the nanocomposite films only released a minuscule amount (21.05 ± 0.054 ppm) of TiO₂ in water. TiO₂ nanoparticles were found in the plasma membrane of epithelial cell line after long-term exposure (10-day) of these cells to large amounts of the free nanoparticles. SSPS/TiO₂ nanocomposites showed excellent antimicrobial activity against Staphylococcus aureus PTCC 1431 (ATCC 25923), while neither anti-cancerous nor pro-cancerous activity was observed for these nanocomposites denoting their neutrality with respect to cancer suppression or progression in gastrointestinal tract. In conclusion, SSPS/TiO₂ nanocomposites could be a promising packaging system for food industries’ objective regarding their physical characteristics, low rate of Ti transition, and low health risk.

在当前的研究中，一组含有不同比例的TiO ₂纳米粒子的可生物降解大豆多糖（SSPS）纳米复合材料被表征为新型包装系统。 X射线衍射(XRD)测量表明，多糖基质中TiO ₂纳米粒子的晶体结构保持完整，并且在扫描电子显微镜(SEM)下观察到含有1％至3％TiO ₂的纳米复合材料的表面形态均匀。动态机械热分析表明，含有 7 wt% 的 SPSS/TiO ₂纳米复合材料的储能模量高出 3.62 倍。与对照SSPS相比，TiO ₂ ％的％表明预期用于食品包装的薄膜的物理性能得到改善。关于这些纳米复合材料的安全性，电感耦合等离子体发射光谱(ICP-OES)表明，在用SSPS/TiO ₂薄膜覆盖并储存6个月的面包样品中没有检测到TiO ₂ 。同样，纳米复合材料薄膜仅在水中释放极少量（21.05 ± 0.054 ppm）的 TiO ₂ 。在上皮细胞系长期暴露于大量游离纳米颗粒（10天）后，在这些细胞的质膜中发现了TiO ₂纳米颗粒。 SSPS/TiO ₂纳米复合材料对金黄色葡萄球菌PTCC 1431 (ATCC 25923) 显示出优异的抗菌活性，而这些纳米复合材料既没有观察到抗癌活性，也没有观察到促癌活性，这表明它们对胃肠道癌症抑制或进展呈中性。 总之，SSPS/TiO ₂纳米复合材料因其物理特性、低Ti转变率和低健康风险而可能成为食品工业目标的有前景的包装系统。