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3D Bioprinting of Self‐Standing Silk‐Based Bioink
Advanced Healthcare Materials ( IF 10.0 ) Pub Date : 2018-01-02 , DOI: 10.1002/adhm.201701026 Zhaozhu Zheng 1 , Jianbing Wu 1 , Meng Liu 2 , Heng Wang 1 , Chunmei Li 3 , María J. Rodriguez 3 , Gang Li 1 , Xiaoqin Wang 1 , David L. Kaplan 3
Advanced Healthcare Materials ( IF 10.0 ) Pub Date : 2018-01-02 , DOI: 10.1002/adhm.201701026 Zhaozhu Zheng 1 , Jianbing Wu 1 , Meng Liu 2 , Heng Wang 1 , Chunmei Li 3 , María J. Rodriguez 3 , Gang Li 1 , Xiaoqin Wang 1 , David L. Kaplan 3
Affiliation
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Silk/polyethylene glycol (PEG) hydrogels are studied as self‐standing bioinks for 3D printing for tissue engineering. The two components of the bioink, silk fibroin protein (silk) and PEG, are both Food and Drug Administration approved materials in drug and medical device products. Mixing PEG with silk induces silk β‐sheet structure formation and thus gelation and water insolubility due to physical crosslinking. A variety of constructs with high resolution, high shape fidelity, and homogeneous gel matrices are printed. When human bone marrow mesenchymal stem cells are premixed with the silk solution prior to printing and the constructs are cultured in this medium, the cell‐loaded constructs maintain their shape over at least 12 weeks. Interestingly, the cells grow faster in the higher silk concentration (10%, w/v) gel than in lower ones (7.5 and 5%, w/v), likely due to the difference in material stiffness and the amount of residual PEG remaining in the gel related to material hydrophobicity. Subcutaneous implantation of 7.5% (w/v) bioink gels with and without printed fibroblast cells in mice reveals that the cells survive and proliferate in the gel matrix for at least 6 week postimplantation. The results suggest that these silk/PEG bioink gels may provide suitable scaffold environments for cell printing and function.
中文翻译：
自立式丝绸基Bioink的3D生物打印
丝绸/聚乙二醇（PEG）水凝胶被研究为用于组织工程3D打印的自立式生物墨水。生物墨水的两个成分，丝素蛋白（丝绸）和PEG，都是食品和药物管理局批准的药品和医疗器械产品中的材料。将PEG与丝绸混合会导致丝绸β片层结构的形成，从而由于物理交联而导致凝胶化和水不溶性。印刷了各种具有高分辨率，高形状保真度和均一凝胶基质的构建体。当在打印之前将人骨髓间充质干细胞与丝溶液预混合，然后在这种培养基中培养构建体时，细胞加载的构建体将保持其形状至少12周。有趣的是，在较高浓度的丝（10％，w / v）凝胶中，细胞的生长比在较低浓度的凝胶中的细胞生长更快（7。5％和5％（w / v），这可能是由于材料刚度的差异以及凝胶中残留的与材料疏水性相关的PEG残留量的差异所致。在小鼠中皮下植入7.5％（w / v）的有和没有印刷成纤维细胞的bioink凝胶表明，这些细胞在植入后至少6周内可以在凝胶基质中存活和增殖。结果表明这些丝绸/ PEG生物墨水凝胶可以为细胞印刷和功能提供合适的支架环境。
更新日期：2018-01-02
中文翻译：
自立式丝绸基Bioink的3D生物打印
丝绸/聚乙二醇（PEG）水凝胶被研究为用于组织工程3D打印的自立式生物墨水。生物墨水的两个成分，丝素蛋白（丝绸）和PEG，都是食品和药物管理局批准的药品和医疗器械产品中的材料。将PEG与丝绸混合会导致丝绸β片层结构的形成，从而由于物理交联而导致凝胶化和水不溶性。印刷了各种具有高分辨率，高形状保真度和均一凝胶基质的构建体。当在打印之前将人骨髓间充质干细胞与丝溶液预混合，然后在这种培养基中培养构建体时，细胞加载的构建体将保持其形状至少12周。有趣的是，在较高浓度的丝（10％，w / v）凝胶中，细胞的生长比在较低浓度的凝胶中的细胞生长更快（7。5％和5％（w / v），这可能是由于材料刚度的差异以及凝胶中残留的与材料疏水性相关的PEG残留量的差异所致。在小鼠中皮下植入7.5％（w / v）的有和没有印刷成纤维细胞的bioink凝胶表明，这些细胞在植入后至少6周内可以在凝胶基质中存活和增殖。结果表明这些丝绸/ PEG生物墨水凝胶可以为细胞印刷和功能提供合适的支架环境。
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