Polymeric Nanocapsules for Enzyme Stabilization in Organic Solvents,Macromolecules

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Polymeric Nanocapsules for Enzyme Stabilization in Organic Solvents
Macromolecules ( IF 5.1 ) Pub Date : 2018-01-02 00:00:00 , DOI: 10.1021/acs.macromol.7b02377
Fumi Ishizuka ₁ , Robert Chapman ₁ , Rhiannon P. Kuchel ₁ , Marion Coureault ₁ , Per B. Zetterlund ₁ , Martina H. Stenzel ₁

Affiliation

Herein we report an approach to encapsulate enzymes within polymeric nanocapsules dispersed in an organic solvent via inverse miniemulsion periphery RAFT polymerization (IMEPP). Glucose oxidase (GOx), which has various applications but is unstable at elevated temperature and in organic solvents, was chosen as a model enzyme. In this study, we have explored the use of photoinitiation under visible (blue) light instead of thermal initiation to avoid enzyme denaturation by heating. GOx was successfully encapsulated within polymeric nanocapsules (∼200 nm) and showed high activity (71–100% relative to free GOx in PBS) dispersed in toluene/t-BuOH. The nanocapsules were thus able to protect GOx and enable it to function in an organic solvent mixture where native GOx would otherwise undergo denaturation. This approach of enzyme encapsulation is significant as it may lead to increased industrial applications of enzyme biocatalysis, expanding the use of enzymes as nontoxic and environmentally friendly biocompatible catalysts.

中文翻译：

用于有机溶剂中酶稳定的聚合物纳米胶囊

在本文中，我们报告了一种通过逆微乳液外围RAFT聚合（IMEPP）将酶包裹在分散在有机溶剂中的聚合物纳米胶囊中的方法。葡萄糖氧化酶（GOx）具有多种用途，但在高温和有机溶剂中不稳定，因此被选为模型酶。在这项研究中，我们探索了在可见（蓝）光下使用光引发代替热引发的方法，以避免酶因加热而变性。GOx成功地封装在聚合物纳米胶囊（〜200 nm）中，并显示出高活性（相对于PBS中游离GOx的71–100％）分散在甲苯/ t中-BuOH。因此，纳米胶囊能够保护GOx并使它能够在有机溶剂混合物中发挥作用，否则天然GOx可能会发生变性。这种酶包封的方法意义重大，因为它可能导致酶生物催化的工业应用增加，扩大酶作为无毒且对环境友好的生物相容性催化剂的用途。

更新日期：2018-01-02

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