A rapid lateral flow immunochromatographic strip (ICS) using fluorescein isothiocyanate (FITC) labeled antigen and antibody was developed for the detection of Acidovorax citrulli (Ac) in melons and vegetable samples. In the ICS, signal amplification was realized based on antigen Ac and anti-Ac monoclonal antibody (McAb) 4F conjugated with FITC, respectively, which were forming two probes. The control line and the test line were obtained by immobilizing the goat anti-mouse IgG antibody and anti-Ac McAb 6D on both sides of the nitrocellulose membrane. The visual detection limit of the strip was 10⁵ CFU/mL, which was 10-fold sensitive compared to the strip of FITC only labeling antigen or antibody. Signal amplification ICS was successfully applied to the detection of Ac in melon and vegetable samples with less detection time and operation procedures compared to the traditional enzyme-linked immunosorbent assay (ELISA) and PCR methods. This is the first report of using FITC labeled antigen and McAb as dual fluorescent probes to develop a direct-type immunofluorescence strip for the rapid and sensitive detection of Ac, which demonstrates a powerful tool for rapidly screening Ac in plant materials and other samples.

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开发了一种使用异硫氰酸荧光素（FITC）标记的抗原和抗体的快速侧流免疫层析带（ICS），用于检测瓜类和蔬菜样品中的柠檬酸瓜氨酸（Ac）。在ICS中，信号放大是基于分别与FITC偶联的抗原Ac和抗Ac单克隆抗体（McAb）4F形成的，这两个探针形成了两个探针。通过将山羊抗小鼠IgG抗体和抗Ac McAb 6D固定在硝酸纤维素膜的两侧获得对照线和测试线。试纸的视觉检测极限为10 ⁵CFU / mL，与仅贴有FITC标记抗原或抗体的条带相比，敏感性为10倍。与传统的酶联免疫吸附测定（ELISA）和PCR方法相比，信号放大ICS已成功应用于瓜类和蔬菜样品中的Ac检测，且检测时间和操作步骤更少。这是首次使用FITC标记的抗原和McAb作为双重荧光探针开发直接型免疫荧光条以快速，灵敏地检测Ac的报道，这证明了一种在植物材料和其他样品中快速筛选Ac的强大工具。

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