The transient isotachophoretic stacking and sweeping was used for the on-line large-volume sample pre-concentration of bacteria, Escherichia coli and Staphylococcus aureus cells (methicillin-susceptible or methicillin-resistant), in the initial stage of micellar electrokinetic chromatography using a non-ionogenic surfactant or of capillary electrophoresis, respectively. These procedures were employed in single-piece fused silica capillary etched with supercritical water with two different internal diameter segments featuring different inner surface roughness. Large volumes (maximum 2.8 μL) of the high conductivity sample matrices, physiological saline solution, urine or blood (with purification step), spiked with examined cells were injected into the wider end of a capillary with an inlet inner diameter 195 μm. This novel on-line combination of preconcentration strategies for cells produced an up to 680-fold increase in sensitivity for E. coli or S. aureus cells. The average calculated resolutions, R, for five selected methicillin-susceptible or methicillin-resistant strains were found to be 6.3 for the agar-cultivated and 14.9 for the blood-incubated cells. A low number of bacteria similar to those in clinical samples were also tested. The modified surface roughness step helped to significantly narrow the cell zones and to increase resolution. The migration velocities of E. coli agar-cultivated and blood-incubated cells were approximately the same as those of S. aureus, probably due to the minimal differences in their surface properties. This procedure, on-line pre-concentration and separation of bacteria, is rapid and provides good reproducibility and repeatability.

瞬时等速电泳叠加和扫描用于细菌，大肠杆菌和金黄色葡萄球菌的在线大体积样品预富集分别在使用非离子源性表面活性剂的胶束电动色谱法或毛细管电泳法的胶束电动色谱法的初始阶段对细胞（对甲氧西林敏感或耐甲氧西林的细胞）这些程序用于用超临界水蚀刻的单片熔融石英毛细管中，该毛细管具有两个不同的内径段，这些内径段具有不同的内表面粗糙度。将大量（最大2.8μL）高电导率样品基质，生理盐水溶液，尿液或血液（带有纯化步骤）（掺有被检查的细胞）注入到毛细管内径较宽的一端，入口内径为195μm。这种细胞预浓缩策略的新型在线组合对大肠杆菌或金黄色葡萄球菌的敏感性提高了多达680倍细胞。发现五个选定的对甲氧西林敏感或耐甲氧西林的菌株的平均计算分辨率R对于培养的琼脂而言是6.3，对于血液培养的细胞而言是14.9。还测试了少量与临床样品中相似的细菌。改进的表面粗糙度步骤有助于显着缩小单元区域并提高分辨率。大肠杆菌琼脂培养细胞和血液培养细胞的迁移速度与金黄色葡萄球菌的迁移速度大致相同，这可能是由于其表面特性的最小差异所致。在线预浓缩和分离细菌的这一过程非常迅速，并提供了良好的重现性和可重复性。