Synthetic cannabinoids in hair – Pragmatic approach for method updates, compound prevalences and concentration ranges in authentic hair samples,Analytica Chimica Acta

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Synthetic cannabinoids in hair – Pragmatic approach for method updates, compound prevalences and concentration ranges in authentic hair samples
Analytica Chimica Acta ( IF 5.7 ) Pub Date : 2018-05-01 , DOI: 10.1016/j.aca.2017.12.029
Florian Franz ₁ , Hanna Jechle ₂ , Verena Angerer ₁ , Melanie Pegoro ₂ , Volker Auwärter ₂ , Merja A Neukamm ₂

Affiliation

Since the first detection of synthetic cannabinoids (SCs) in so-called 'legal high' products (e.g. 'Spice') sold as legal alternatives to marihuana, the rapid development of this class of designer drugs poses a great challenge for analytical laboratories. The aim of this study was the comprehensive validation of an up-to-date LC-MS/MS method for detection of SCs in human hair for the purpose of drug abstinence testing and evaluation of a pragmatic re-validation approach for frequent method adaption. The validation demonstrated low quantification limits (0.5-5.0 pg mg-1) and acceptable selectivity, linearity, accuracy, and precision for 72 SCs. High matrix effects have been taken into consideration as a major limitation of the method. The partial re-validation approach proved to be an appropriate compromise between reduced validation effort and sufficient control of the method performance enabling analysts to keep pace with the dynamics of the drug market. The analysis of 294 authentic samples resulted in 163 positive samples and showed a broad concentration range (<1.0-5,700 pg mg-1) for 52 SCs in hair with up to 17 different compounds detected in a single hair sample. Periods of detection between one and 58 months were observed for single compounds in hair. Regarding the interpretation of analytical findings semi-quantitative concentrations were considered sufficient for a rough classification of the intensity of drug exposure in (i) passive exposure or exposure in the distant past (lower pg mg-1 range), (ii) more intense exposure (elevated concentration range, >20 pg mg-1 (upper 25th-percentile)), and (iii) heavy/recent exposure (>150 pg mg-1).

中文翻译：

头发中的合成大麻素——真实头发样品中方法更新、化合物流行率和浓度范围的务实方法

自从首次在作为大麻合法替代品出售的所谓“合法高”产品（例如“香料”）中检测到合成大麻素（SC）以来，此类设计药物的快速发展对分析实验室提出了巨大的挑战。本研究的目的是对用于检测人类头发中 SC 的最新 LC-MS/MS 方法进行全面验证，以进行戒毒测试，并评估用于频繁方法调整的实用重新验证方法。验证证明了 72 个 SC 的定量限较低（0.5-5.0 pg mg-1）和可接受的选择性、线性、准确度和精密度。高基体效应已被认为是该方法的主要限制。事实证明，部分重新验证方法是减少验证工作和充分控制方法性能之间的适当折衷，使分析师能够跟上药品市场的动态。对 294 个真实样品的分析得出了 163 个阳性样品，并显示出广泛的浓度范围（<1 id=4>20 pg mg-1（上 25 个百分位数）），以及 (iii) 重度/近期暴露（>150 pg mg） -1）。

更新日期：2018-05-01

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