Probe for dual-site target distinct subcellular compartments from cytosol and nucleus is an attractive approach, however, which was scarcely reported. Herein, a series of small-molecular thiophene pyridium salt derivatives (MitoNuc1-4) possessing water-soluble, high quantum yield and two-photon activity were rationally designed, and their structures were crystallographic confirmed. Systematic photophysical and biological imaging property investigations were carried out for them. It was found that MitoNuc1-4 exhibit two-photon absorption properties in the near infrared region, and MitoNuc1 has membrane permeability and cationic nature, rendering it to be double labelling of mitochondria and nucleolus in living cells with superb photo-stability and non-invasiveness. It also demonstrated that MitoNuc1 in living cells can monitor mitochondrial division in real time and revealed nucleolar ultrastructure under stimulated emission depletion nanoscopy.

中文翻译：

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高量子产率噻吩吡啶盐生物传感器对细胞内线粒体和核仁的双重标记及其在受激发射耗尽纳米学中的应用

然而，从细胞质和细胞核中探测双位点目标不同亚细胞区室是一种有吸引力的方法，但鲜有报道。在此，合理设计了一系列具有水溶性、高量子产率和双光子活性的小分子噻吩吡啶盐衍生物（MitoNuc1-4），并对其结构进行了晶体学确证。对它们进行了系统的光物理和生物成像特性研究。发现MitoNuc1-4在近红外区域表现出双光子吸收特性，并且MitoNuc1具有膜渗透性和阳离子性，使其成为活细胞中线粒体和核仁的双重标记，具有极好的光稳定性和非侵入性.