Overexpression of the Anthocyanidin Synthase Gene in Strawberry Enhances Antioxidant Capacity and Cytotoxic Effects on Human Hepatic Cancer Cells,Journal of Agricultural and Food Chemistry

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Overexpression of the Anthocyanidin Synthase Gene in Strawberry Enhances Antioxidant Capacity and Cytotoxic Effects on Human Hepatic Cancer Cells
Journal of Agricultural and Food Chemistry ( IF 5.7 ) Pub Date : 2018-01-08 00:00:00 , DOI: 10.1021/acs.jafc.7b04177
Francesca Giampieri , Massimiliano Gasparrini , Tamara Y. Forbes-Hernandez ₁ , Luca Mazzoni , Franco Capocasa , Silvia Sabbadini , Josè M. Alvarez-Suarez ₂ , Sadia Afrin , Carlo Rosati ₃ , Tiziana Pandolfini ₄ , Barbara Molesini ₄ , José F. Sánchez-Sevilla ₅ , Iraida Amaya ₅ , Bruno Mezzetti , Maurizio Battino ₆

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Food fortification through the increase and/or modulation of bioactive compounds has become a major goal for preventing several diseases, including cancer. Here, strawberry lines of cv. Calypso transformed with a construct containing an anthocyanidin synthase (ANS) gene were produced to study the effects on anthocyanin biosynthesis, metabolism, and transcriptome. Three strawberry ANS transgenic lines (ANS L5, ANS L15, and ANS L18) were analyzed for phytochemical composition and total antioxidant capacity (TAC), and their fruit extracts were assessed for cytotoxic effects on hepatocellular carcinoma. ANS L18 fruits had the highest levels of total phenolics and flavonoids, while those of ANS L15 had the highest anthocyanin concentration; TAC positively correlated with total polyphenol content. Fruit transcriptome was also specifically affected in the polyphenol biosynthesis and in other related metabolic pathways. Fruit extracts of all lines exerted cytotoxic effects in a dose/time-dependent manner, increasing cellular apoptosis and free radical levels and impairing mitochondrial functionality.

中文翻译：

草莓中花青素合酶基因的过表达增强抗氧化能力和对人肝癌细胞的细胞毒性作用

通过增加和/或调节生物活性化合物来强化食品已成为预防几种疾病（包括癌症）的主要目标。在这里，草莓行的简历。产生了用含有花色素苷合酶（ANS）基因的构建体转化的Calypso，以研究其对花色素苷生物合成，代谢和转录组的影响。三草莓ANS分析转基因品系（ANS L5，ANS L15和ANS L18）的植物化学组成和总抗氧化能力（TAC），并评估其果实提取物对肝细胞癌的细胞毒性作用。ANS L18果实中的总酚和类黄酮含量最高，而ANS L15的花色苷浓度最高。TAC与总多酚含量呈正相关。水果转录组还受到多酚生物合成和其他相关代谢途径的特异性影响。所有品系的果实提取物均以剂量/时间依赖性方式发挥细胞毒性作用，从而增加细胞凋亡和自由基水平，并损害线粒体功能。

更新日期：2018-01-08

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