Absence of Albumin Improves in Vitro Cellular Uptake and Disruption of Poloxamer 407-Based Nanoparticles inside Cancer Cells,Molecular Pharmaceutics

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Absence of Albumin Improves in Vitro Cellular Uptake and Disruption of Poloxamer 407-Based Nanoparticles inside Cancer Cells
Molecular Pharmaceutics ( IF 4.5 ) Pub Date : 2018-01-11 00:00:00 , DOI: 10.1021/acs.molpharmaceut.7b00893
Ana Loureiro ₁ , Jennifer Noro ₁ , Ana S. Abreu ₂ , Eugénia Nogueira ₁ , Diana Soares da Costa _{3,

4} , Carla Silva ₁ , Artur Cavaco-Paulo ₁

Affiliation

Novel nanoparticles based on Poloxamer 407 and vegetable oil were produced by high pressure homogenization. Functionalization of those nanoparticles was made by incorporation of folic acid (FA)–Poloxamer 407 conjugate. These nanoparticles showed suitable characteristics for intravenous therapeutic applications similarly to PEGylated albumin-based nanoparticles, previously described by our research group. Here, we found that the absence of albumin at the interface of Poloxamer 407-based nanoparticles improves the overall process of in vitro cellular uptake and nanoparticle disruption inside cancer cells (folate receptor, FR, positive cells). The results presented here suggest that interfacial composition of those nanoparticles is of paramount importance for drug trafficking inside cancer cells.

中文翻译：

缺乏白蛋白可提高癌细胞内基于泊洛沙姆407的纳米颗粒的体外细胞摄取和破坏。

通过高压均质化生产了基于泊洛沙姆407和植物油的新型纳米颗粒。这些纳米颗粒的功能化是通过掺入叶酸（FA）-Poloxamer 407共轭物来实现的。这些纳米颗粒显示出适合静脉治疗应用的特性，类似于我们的研究小组先前描述的基于聚乙二醇化白蛋白的纳米颗粒。在这里，我们发现基于泊洛沙姆407的纳米颗粒界面处缺乏白蛋白改善了癌细胞（叶酸受体，FR，阳性细胞）中体外细胞摄取和纳米颗粒破坏的总体过程。此处给出的结果表明，这些纳米颗粒的界面组成对于癌细胞内的药物运输至关重要。

更新日期：2018-01-11

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