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An OPR3-independent pathway uses 4,5-didehydrojasmonate for jasmonate synthesis
Nature Chemical Biology ( IF 12.9 ) Pub Date : 2018-01-01 , DOI: 10.1038/nchembio.2540
Andrea Chini , Isabel Monte , Angel M Zamarreño , Mats Hamberg , Steve Lassueur , Philippe Reymond , Sally Weiss , Annick Stintzi , Andreas Schaller , Andrea Porzel , José M García-Mina , Roberto Solano

Biosynthesis of the phytohormone jasmonoyl-isoleucine (JA-Ile) requires reduction of the JA precursor 12-oxo-phytodienoic acid (OPDA) by OPDA reductase 3 (OPR3). Previous analyses of the opr3-1 Arabidopsis mutant suggested an OPDA signaling role independent of JA-Ile and its receptor COI1; however, this hypothesis has been challenged because opr3-1 is a conditional allele not completely impaired in JA-Ile biosynthesis. To clarify the role of OPR3 and OPDA in JA-independent defenses, we isolated and characterized a loss-of-function opr3-3 allele. Strikingly, opr3-3 plants remained resistant to necrotrophic pathogens and insect feeding, and activated COI1-dependent JA-mediated gene expression. Analysis of OPDA derivatives identified 4,5-didehydro-JA in wounded wild-type and opr3-3 plants. OPR2 was found to reduce 4,5-didehydro-JA to JA, explaining the accumulation of JA-Ile and activation of JA-Ile-responses in opr3-3 mutants. Our results demonstrate that in the absence of OPR3, OPDA enters the β-oxidation pathway to produce 4,5-ddh-JA as a direct precursor of JA and JA-Ile, thus identifying an OPR3-independent pathway for JA biosynthesis.



中文翻译:

不依赖OPR3的途径使用4,5-二氢氢化茉莉酮酸酯进行茉莉酸酯合成

植物激素茉莉基异亮氨酸(JA-Ile)的生物合成需要通过OPDA还原酶3(OPR3)还原JA前体12-氧代-植物二烯酸(OPDA)。先前对opr3-1拟南芥突变体的分析表明,OPDA信号传导作用独立于JA-Ile及其受体COI1。然而,该假设受到质疑,因为opr3-1是JA-Ile生物合成中未完全受损的条件等位基因。为了阐明OPR3和OPDA在不依赖JA的防御中的作用,我们分离并鉴定了功能丧失的opr3-3等位基因。令人惊讶的是opr3-3植物对坏死性病原体和昆虫摄食仍然具有抗性,并激活了依赖COI1的JA介导的基因表达。OPDA衍生物的分析确定了受伤的野生型和opr3-3植物中的4,5-didehydro-JA 。发现OPR2将4,5-didehydro-JA还原为JA,解释了opr3-3突变体中JA-Ile的积累和JA-Ile反应的激活。我们的结果表明,在不存在OPR3的情况下,OPDA进入β-氧化途径,产生4,5-ddh-JA作为JA和JA-Ile的直接前体,从而确定了JA生物合成的不依赖OPR3的途径。

更新日期:2018-01-01
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