Dual Amplified Electrochemical Immunosensor for Hepatitis B Virus Surface Antigen Detection Using Hemin/G‐Quadruplex Immobilized onto Fe3O4‐AuNPs or (Hemin‐Amino‐rGO‐Au) Nanohybrid,Electroanalysis

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Dual Amplified Electrochemical Immunosensor for Hepatitis B Virus Surface Antigen Detection Using Hemin/G‐Quadruplex Immobilized onto Fe3O4‐AuNPs or (Hemin‐Amino‐rGO‐Au) Nanohybrid
Electroanalysis ( IF 2.7 ) Pub Date : 2017-12-29 , DOI: 10.1002/elan.201700727
Negar Alizadeh ₁ , Rahman Hallaj _{1,

2} , Abdollah Salimi _{1,

2}

Affiliation

A sensitive electrochemical immunosensor for Hepatitis B virus surface antigen (HBsAg) detection was fabricated based on hemin/G‐quadruplex interlaced onto Fe₃O₄‐AuNPs or hemin ‐amino‐reduced graphene oxide nanocomposite (H‐amino‐rGO‐Au). G‐quadruplex DNAzyme, which is composed of hemin and guanine‐rich nucleic acid, is an effective signal amplified tool for its outstanding peroxidase activity and Fe₃O₄‐AuNPs or (H‐amino‐rGO‐Au) nanocomposites with quasi‐enzyme activity provide appropriate support for the immobilization of hemin/G‐quadruplex. The target protein was sandwiched between the primary antibody immobilized on the GO and secondary antibody immobilized on the Fe₃O₄‐AuNPs or (H‐amino‐rGO‐Au) nanocomposites and glutaraldehyde was used as linking agent for the immobilization of primary antibody on the surface of GO. Both Fe₃O₄‐AuNPs and H‐amino‐rGO‐Au nanocomposite and also hemin/G‐quadruplex can cooperate the electrocatalytic reduction of H₂O₂ in the presence of methylene blue as mediator. The proposed immunosensor has a wide linear dynamic range of 0.1 pg/ml to 300 pg/ml with a detection limit of 60 fg/ml when Fe₃O₄‐AuNPs was used for immobilization of hemin/G‐quadruplex, while the dynamic range and DL were 0. 1–1000 pg/mL and 10 fg/mL, respectively in the presence of H‐amino‐rGO‐ Au nanocomposite as platform for immobilizing of hemin/G‐quadruplex. The proposed immunosensor was also used for analysis of HBsAg in spiked human serum samples with satisfactory results.

中文翻译：

使用固定在Fe3O4-AuNPs或（Hemin-Amino-rGO-Au）纳米杂交体上的Hemin / G-Quadruplex检测乙肝病毒表面抗原的双放大电化学免疫传感器

基于在Fe ₃ O ₄ -AuNPs或hemin-氨基还原石墨烯氧化物纳米复合材料（H-amino-rGO-Au）上交织的hemin / G-quadruplex制备了用于检测乙型肝炎病毒表面抗原（HBsAg）的灵敏电化学免疫传感器。G-四链体DNA酶由富含血红素和鸟嘌呤的核酸组成，是一种有效的信号放大工具，具有出色的过氧化物酶活性以及Fe ₃ O ₄ -AuNPs或（H-amino-rGO-Au）纳米复合物与准酶的结合活性为固定血红素/ G-四链体提供了适当的支持。将目标蛋白夹在固定在GO上的一抗和固定在Fe ₃ O ₄上的二抗之间-AuNPs或（H-氨基-rGO-Au）纳米复合材料与戊二醛被用作将一抗固定在GO表面的连接剂。Fe ₃ O ₄ -AuNPs和H-amino-rGO-Au纳米复合材料以及hemin / G-quadruplex都可以在亚甲基蓝作为介体存在下协同催化H ₂ O ₂的电催化还原。所提出的免疫传感器具有0.1 pg / ml至300 pg / ml的宽线性动态范围，当Fe ₃ O ₄时的检出限为60 fg / ml。‐AuNPs用于固定血红素/ G-四链体，而动态范围和DL分别为0. 1–1000 pg / mL和10 fg / mL，在存在H‐氨基‐rGO‐Au纳米复合材料作为平台的情况下固定血红素/ G-四链体。所提出的免疫传感器也用于分析加标的人血清样品中的HBsAg，结果令人满意。

更新日期：2017-12-29

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