Nanoparticles have been widely used in several sectors and their long-term effect on the body and environment remains unknown. To evaluate the mutagenic, recombinogenic and carcinogenic potential of 11 nm titanium dioxide nanocrystals (TiO2 NCs), the Somatic Mutation and Recombination Test (SMART) and the Test for Detection of Epithelial Tumors Clones (Warts-Wts) were used, both in Drosophila melanogaster. Third-instar larvae (72 + 4 h), obtained in both tests, were treated with different concentrations of TiO2 NCs ranging from 6.25 to 100 mM. Ultrapure water and urethane were used as negative and positive controls, respectively. At ST cross, all concentrations of TiO2 NCs showed a significant increase in the frequencies of mutant spots, demonstrating higher recombination rates. At the HB cross, only the 50 mM concentration showed a negative result. In the Wts Test, all used concentrations were carcinogenic, except for the 100 mM one, which was toxic. No relationship was demonstrated between the used concentrations and the obtained responses. There was no interference of the cytochrome P450 enzyme complex in the induction of mutant spots.

纳米颗粒已在多个领域广泛使用，它们对人体和环境的长期影响仍然未知。为了评估11 nm二氧化钛纳米晶体（TiO2 NCs）的诱变，重组和致癌潜力，在果蝇中使用了体细胞突变和重组测试（SMART）和上皮肿瘤克隆检测（Warts-Wts）。 。在两个测试中获得的三龄幼虫（72 + 4小时）用浓度范围为6.25至100 mM的不同浓度的TiO2 NCs处理。超纯水和尿烷分别用作阴性和阳性对照。在ST交叉点，所有浓度的TiO2 NCs都显示出突变点的频率显着增加，表明了更高的重组率。在HB十字路口，仅50 mM浓度显示阴性结果。在Wts测试中，除100 mM具有毒性外，所有使用的浓度均具有致癌性。所使用的浓度与所获得的响应之间没有关系。细胞色素P450酶复合物在诱变斑点的诱导中没有干扰。