Comfrey root preparations are used for the external treatment of joint distortions and myalgia, due to its analgesic and anti-inflammatory properties. Up to date, key activity-determining constituents of comfrey root extracts have not been completely elucidated. Therefore, we applied different approaches to further characterize a comfrey root extract (65% ethanol). The phenolic profile of comfrey root sample was characterized by HPLC-DAD-QTOF-MS/MS. Rosmarinic acid was identified as main phenolic constituent (7.55 mg/g extract). Moreover, trimers and tetramers of caffeic acid (isomers of salvianolic acid A, B and C) were identified and quantified for the first time in comfrey root. In addition, pyrrolizidine alkaloids were evaluated by HPLC-QQQ-MS/MS and acetylintermedine, acetyllycopsamine and their N-oxides were determined as major pyrrolizidine alkaloids in the comfrey root sample. Lastly, the antioxidant activity was determined using four assays: DPPH and ABTS radicals scavenging assays, reducing power assay and 15-lipoxygenase inhibition assay. Comfrey root extract exhibited significant antioxidant activities when compared to known antioxidants. Thus, comfrey root is an important source of phenolic compounds endowed with antioxidant activity which may contribute to the overall bioactivity of Symphytum preparations.

由于其止痛和抗炎特性，紫f根制剂可用于关节畸形和肌痛的外部治疗。迄今为止，还没有完全阐明菊苣根提取物的决定活性的关键成分。因此，我们应用了不同的方法进一步表征了紫f根提取物（65％的乙醇）。通过HPLC-DAD-QTOF-MS / MS表征了紫草根样品的酚类谱。迷迭香酸被鉴定为主要的酚类成分（7.55 mg / g提取物）。此外，咖啡因酸的三聚体和四聚体（丹酚酸A，B和C的异构体）首次在紫花苜蓿根中得到鉴定和定量。此外，通过HPLC-QQQ-MS / MS和乙酰基中间体对吡咯嗪核生物碱进行了评估，乙酰基胭脂红胺及其N-氧化物被确定为康弗莱根样品中的主要吡咯烷核生物碱。最后，使用四种测定法测定抗氧化剂活性：DPPH和ABTS自由基清除测定法，还原能力测定法和15-脂氧合酶抑制测定法。与已知的抗氧化剂相比，草根提取物表现出显着的抗氧化剂活性。因此，紫f根是酚类化合物的重要来源，其具有抗氧化活性，可能有助于植物的整体生物活性。共生制剂。