The use of essential oils (EOs) is known since long time in traditional medicine and aromatherapy for the management of various oxidative stress-related disorders and has been further increased recently for their neuroprotective and anti-aging potentials as well as for reducing anxiety and stress.

The purpose of this work was to evaluate, for the first time, the chemical composition of Citrus lumia Risso EO and its antioxidant, anti-cholinesterase, and neuroactive properties by cell-free and cell-based assays.

The EO has shown strong antioxidant and free radical scavenging properties, particularly in hydrogen atom transfer based assays (β-carotene bleaching and ORAC, IC₅₀ 22 μg/mL and 46 μg/mL, respectively), that can be attributed to the high content of monoterpenes, especially d-Limonene (48.905%), and Linalool (18.245%). Furthermore, the EO has shown an interesting anti-acetylcholinesterase activity (IC₅₀ 258.25 μg/mL). Data from MTT analysis indicate that the cytotoxicity of EO, evaluated on L929 mouse fibroblasts, is very low, with an IC₅₀ higher than 500 μg/mL at 48 h. Rat neuronal networks subjected to EO showed a concentration-dependent inhibition of spontaneous electrical activity.

Results indicate that C. lumia EO could be an important source of natural antioxidants suggesting an important preventive role in the onset of oxidative stress-related pathologies.

香精油（EOs）的使用在传统医学和芳香疗法中长期以来广为人知，可用于管理各种与氧化应激相关的疾病，近来由于其神经保护和抗衰老潜力以及减轻焦虑和压力而进一步增加。

这项工作的目的是通过无细胞和基于细胞的测定法，首次评估柑桔（Citrus lumia Risso）EO的化学成分及其抗氧化剂，抗胆碱酯酶和神经活性。

EO具有很强的抗氧化剂和自由基清除性能，尤其是在基于氢原子转移的测定中（β-胡萝卜素漂白和ORAC，IC ₅₀分别为22μg/ mL和46μg/ mL），这可以归因于高含量单萜，尤其是d-柠檬烯（48.905％）和芳樟醇（18.245％）。此外，EO还显示出令人感兴趣的抗乙酰胆碱酯酶活性（IC ₅₀ 258.25μg/ mL）。来自MTT分析的数据表明，在L929小鼠成纤维细胞上评估的EO的细胞毒性非常低，在48 h时IC ₅₀高于500μg/ mL。经历EO的大鼠神经元网络显示出自发性电活动的浓度依赖性抑制作用。

结果表明，C。lumia EO可能是天然抗氧化剂的重要来源，表明在氧化应激相关疾病发作中具有重要的预防作用。