Pomegranate (Punica granatum) is a heath with abundant hydrolysable tannin in its peel. This study demonstrated that POMx (70% acetone extract of pomegranate peels) significantly reduced interleukin (IL)-1β-induced inducible nitric oxide synthase, cyclooxygenase-2, and matrix metalloproteinase-13 protein expression in primary rat chondrocytes (PRCs). In the type II collagenase-induced osteoarthritis rat model, POMx (150 mg/kg) recovered the weight-bearing ratio changes in experimental animals, suggesting the alleviation of knee-related inflammation and nociception. Therefore, the major compound, punicalagin, was isolated from POMx. Punicalagin also significantly reduced IL-1β-induced inflammatory factors in PRCs and exerted significant antiosteoarthritis effects in the vivo model after 28-d treatment (concentration: 0.50 mg/kg). However, the IC₅₀ values of POMx and punicalagin against prostaglandin E₂ production were 83.2 and 36.0 μg/mL, respectively, and the concentration of punicalagin in POMx was 19.1%. Altogether, POMx can be used to develop functional foods for knee-related diseases, and punicalagin can act as an active ingredient.

石榴（石榴））是一种果皮，其果皮中含有大量可水解的单宁酸。这项研究表明POMx（石榴皮的70％丙酮提取物）显着降低白细胞介素（IL）-1β诱导的原代大鼠软骨细胞（PRC）中的诱导型一氧化氮合酶，环氧合酶2和基质金属蛋白酶13蛋白质表达。在II型胶原酶诱发的骨关节炎大鼠模型中，POMx（150 mg / kg）恢复了实验动物的体重比变化，表明膝相关的炎症和伤害感受得到缓解。因此，主要化合物punicalagin是从POMx中分离出来的。在28天治疗后（浓度：0.50 mg / kg），普尼卡拉金还显着减少了PRCs中IL-1β诱导的炎性因子，并在体内模型中发挥了显着的抗骨关节炎作用。但是，IC ₅₀POMx和punicalagin对前列腺素E ₂产生的最高值分别为83.2和36.0μg / mL，POMx中的punicalagin浓度为19.1％。总而言之，POMx可用于开发与膝盖相关疾病的功能性食品，而punicalagin可作为有效成分。