The contamination of the environment by U may affect plant life and consequently may have an impact on animal and human health. The present work describesU(VI) sequestration by Acidovorax facilis using a multidisciplinary approach combining wet chemistry, transmission electron microscopy, and spectroscopy methods (e.g. cryo-time resolved laser-induced fluorescence spectroscopy, extended X-ray absorption fine structure spectroscopy, and in-situ attenuated total reflection Fourier transform infrared spectroscopy). This bacterial strain is widely distributed in nature including U-contaminated sites. In kinetic batch experiments cells of A. facilis were contacted for 5 min to 48 h with 0.1 mM U(VI). The results show that the local coordination of U species associated with the cells depends upon time contact. U is bound mainly to phosphate groups of lipopolysaccharide (LPS) at the outer membrane within the first hour. And, that both, phosphoryl and carboxyl functionality groups of LPS and peptidoglycan of A. facilis cells may effectuate the removal of high U amounts from solution at 24–48 h of incubation. It is clearly demonstrated that A. facilis may play an important role in predicting the transport behaviour of U in the environment and that the results will contribute to the improvement of bioremediation methods of U-contaminated sites.

铀对环境的污染可能会影响植物的生命，因此可能对动物和人类健康产生影响。由本工作describesU（VI）螯合敏捷食酸facilis使用多学科方法相结合的湿化学，透射电子显微镜，和光谱学方法（例如冷冻时间分辨激光诱导荧光光谱法，扩展X射线吸收精细结构谱，和IN-原位衰减全反射傅立叶变换红外光谱）。该细菌菌株在自然界中广泛分布，包括受U污染的部位。在动力学分批实验中，A。facilis细胞将其与0.1 mM U（VI）接触5分钟至48小时。结果表明，与细胞相关的U物种的局部协调取决于时间接触。在第一小时内，U主要与外膜的脂多糖（LPS）的磷酸基团结合。而且，LPS的磷酰基和羧基官能团以及农杆菌细胞的肽聚糖都可能在孵育24-48 h时从溶液中去除大量的U。清楚地表明，A。facilis可能在预测U在环境中的运输行为中起重要作用，其结果将有助于改善U污染地点的生物修复方法。