The combination of fluorescent (FL) probe and photosensitizer (PS) is a powerful tool for monitoring the changes of specific organelles while studying the photodynamic therapy (PDT) processes at the organelle level. It is very valued for biomedical application but remained challenging due to the lack of ideal FL PDT agent. In this work, an aggregation-induced emission (AIE)-active FL probe (AIE-FR-TPP) by conjugation of a far red/near-infrared (FR/NIR) emitting AIE fluorogen with the mitochondria-targeting group, is reported as a multi-functional fluorophore for mitochondrial imaging and PDT. As for bioimaging, AIE-FR-TPP can selectively light up the cellular mitochondria with low working concentration, short staining time and good biocompatibility. As for PDT, the probe can target mitochondria precisely and generate reactive oxygen species (ROS) efficiently both in living cells and zebrafish embryos. Moreover, AIE-FR-TPP is capable of monitoring the morphological changes of mitochondria based on the AIE effect. These results demonstrate that a real-time visual PDT process at the organelle level in living zebrafish embryos is achieved for the first time by using the AIE PS to induce mitochondrial ROS collapse and report the morphological change on site and in time.

荧光（FL）探针和光敏剂（PS）的组合是在研究细胞器水平的光动力疗法（PDT）过程的同时，监视特定细胞器变化的强大工具。它在生物医学应用中非常有价值，但由于缺乏理想的FL PDT试剂而仍然具有挑战性。在这项工作中，报道了通过线粒体靶向基团结合发射远红外/近红外（FR / NIR）的发射AIE氟的聚集诱导发射（AIE）活性FL探针（AIE-FR-TPP）作为用于线粒体成像和PDT的多功能荧光团。对于生物成像，AIE-FR-TPP可以选择性地点亮细胞线粒体，工作浓度低，染色时间短且生物相容性好。至于PDT，该探针可以精确地靶向线粒体，并在活细胞和斑马鱼胚胎中高效产生活性氧（ROS）。此外，AIE-FR-TPP能够基于AIE效应监测线粒体的形态变化。这些结果表明，通过使用AIE PS诱导线粒体ROS塌陷并实时报告形态变化，首次实现了活斑马鱼胚胎中细胞器水平的实时视觉PDT过程。