Fluorescent polymeric nanoparticles (FPNs) as novel probes have attracted more and more attention for biomedical applications owing to their optical properties and multifunctional potential. However, most of FPNs based on conventional organic fluorogens encounter serious aggregation-caused quenching (ACQ) effect. In this work, to overcome these problems, amphiphilic fluorescent copolymers [Poly(PEGY-PhE)] containing aggregation-induced emission (AIE) fluorogens (AIEgens) have been prepared via a facile amino-yne click polymerization under rather mild experimental conditions (catalyst-free and room temperature). The Poly(PEGY-PhE) copolymers can self-assemble into FPNs and be well dispersed in aqueous solution. Fluorescence spectra demonstrate that Poly(PEGY-PhE) FPNs possess strong red fluorescence, excellent photostability and low critical micelle concentration. The biological assays confirm that these AIE-active Poly(PEGY-PhE) FPNs possess low cytotoxicity towards L929 cells and cell dyeing performance. More importantly, this amino-yne click polymerization provides a simple strategy to prepare robust biocompatible fluorescent nanoparticles for biological imaging because of their high efficiency, mild reaction conditions and simple experimental operation procedure.

荧光聚合物纳米颗粒（FPN）作为新型探针，由于其光学性质和多功能潜力，已在生物医学应用中吸引了越来越多的关注。但是，大多数基于常规有机氟的FPN都遇到严重的聚集引起的猝灭（ACQ）效应。在这项工作中，为克服这些问题，在相当温和的实验条件下（催化剂），通过简便的氨基炔点击聚合制备了含有聚集诱导发射（AIE）氟原子（AIEgens）的两亲性荧光共聚物[Poly（PEGY-PhE）]。 -和室温）。聚（PEGY-PhE）共聚物可以自组装成FPN，并且可以很好地分散在水溶液中。荧光光谱表明，聚（PEGY-PhE）FPN具有强烈的红色荧光，优异的光稳定性和低的临界胶束浓度。生物学分析证实，这些具有AIE活性的聚（PEGY-PhE）FPN对L929细胞和细胞染色性能具有较低的细胞毒性。更重要的是，这种氨基-炔-点击聚合提供了一种简单的策略来制备用于生物成像的坚固的生物相容性荧光纳米粒子，因为它们的效率高，反应条件温和且实验操作步骤简单。