Multiplexed Profiling of Single Extracellular Vesicles,ACS Nano

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Multiplexed Profiling of Single Extracellular Vesicles
ACS Nano ( IF 15.8 ) Pub Date : 2018-01-04 00:00:00 , DOI: 10.1021/acsnano.7b07060
Kyungheon Lee ₁ , Kyle Fraser ₁ , Bassel Ghaddar ₁ , Katy Yang ₁ , Eunha Kim ₁ , Leonora Balaj ₂ , E Antonio Chiocca ₃ , Xandra O Breakefield ₂ , Hakho Lee ₁ , Ralph Weissleder _{1,

2,

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Affiliation

Extracellular vesicles (EV) are a family of cell-originating, membrane-enveloped nanoparticles with diverse biological function, diagnostic potential, and therapeutic applications. While EV can be abundant in circulation, their small size (∼4 order of magnitude smaller than cells) has necessitated bulk analyses, making many more nuanced biological explorations, cell of origin questions, or heterogeneity investigations impossible. Here we describe a single EV analysis (SEA) technique which is simple, sensitive, multiplexable, and practical. We profiled glioblastoma EV and discovered surprising variations in putative pan-EV as well as tumor cell markers on EV. These analyses shed light on the heterogeneous biomarker profiles of EV. The SEA technology has the potential to address fundamental questions in vesicle biology and clinical applications.

中文翻译：

单个细胞外囊泡的多重分析

细胞外囊泡 (EV) 是一类具有多种生物学功能、诊断潜力和治疗应用的细胞来源、膜包裹的纳米颗粒。虽然 EV 可以在循环中丰富，但它们的小尺寸（比细胞小约 4 个数量级）需要进行大量分析，这使得许多更细微的生物学探索、细胞起源问题或异质性研究成为不可能。在这里，我们描述了一种简单、灵敏、可复用且实用的单一 EV 分析 (SEA) 技术。我们分析了胶质母细胞瘤 EV，并发现了假定的泛 EV 以及 EV 上的肿瘤细胞标志物的惊人变化。这些分析揭示了 EV 的异质生物标志物谱。SEA 技术有可能解决囊泡生物学和临床应用中的基本问题。

更新日期：2018-01-04

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