We conducted a comparative in vitro study on the proliferative effects of natural human lactoferrin (nhLF) and bovine lactoferrin (bLF) on osteoblasts. We investigated cell proliferation, cell survival, cell cycle, and mRNA and protein expression of proliferating cell nuclear antigen. Results indicated that treatment with 100 μg/mL of bLF or nhLF promoted the proliferation and sustenance of osteoblasts, and increased the length of the G₂/M and S phases compared with the untreated osteoblasts. Results of real-time quantitative PCR and Western blot showed that mRNA and protein expression of proliferating cell nuclear antigen by osteoblasts treated with bLF or nhLF were greater than those of the untreated control. At the same concentration, bLF demonstrated a greater effect on osteoblast proliferation than did nhLF. This study provides insights of significance in the utlization of bLF in healthy food formulas.

我们对天然人乳铁蛋白（nhLF）和牛乳铁蛋白（bLF）对成骨细胞的增殖作用进行了体外比较研究。我们调查了细胞增殖，细胞存活，细胞周期以及增殖细胞核抗原的mRNA和蛋白质表达。结果表明，用100μg/ mL的bLF或nhLF处理可促进成骨细胞的增殖和维持，并增加G ₂的长度/ M和S期与未处理的成骨细胞相比。实时定量PCR和Western印迹的结果表明，用bLF或nhLF处理的成骨细胞增殖的细胞核抗原的mRNA和蛋白表达均高于未处理的对照。在相同浓度下，bLF对成骨细胞增殖的影响要大于nhLF。这项研究为健康食品配方中的bLF的利用提供了重要的见解。