Abstract In this present investigation, lipid extraction was performed in marine macro algae Ulva fasciata using soxhlet and super critical fluid extraction apparatus. The ultrasonic pretreatment was done for the algal biomass. Extraction parameters for High extraction yield of algal oil were obtained at 5% moisture content, 0.25 mm particle size, 90 °C, 180 min time, 5:1 solvent-to-solid ratio and solvent flow rate 1 l/hr. After the optimization process 9.85% of oil extraction was obtained from Ulva fasicata algal biomass. The aromatic groups, indicating the presence of 12-Hydroxy-9-octadecenoic acid was confirmed by FT-IR analysis. Thus the algal oil obtained from Ulva fasicata biomass can be used for the biodiesel production. The saturated fatty acid (SFAs) was found to be 75.43% in the produced algal oil. The properties like Acid value, Iodine number, specific gravity, Saponification value, calorific value, Flash point, Fire point and kinematic viscosity was also characterized.

中文翻译：

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一种从海洋大型藻类石莼中提取藻油的新方法

摘要 在本研究中，使用索氏和超临界流体提取装置在海洋巨藻石莼中进行脂质提取。对藻类生物质进行超声波预处理。在 5% 水分含量、0.25 毫米粒径、90 °C、180 分钟时间、5:1 溶剂与固体比和溶剂流速 1 l/hr 下获得了藻油高提取率的提取参数。在优化过程之后，9.85% 的油提取物从 Ulva fasicata 藻类生物质中获得。FT-IR 分析证实了芳香族基团，表明存在 12-羟基-9-十八碳烯酸。因此，从Ulva fasicata 生物质中获得的藻油可用于生产生物柴油。发现所生产的藻油中的饱和脂肪酸 (SFAs) 为 75.43%。酸值等特性，