The absorption kinetics of food ingredients such as nanoemulsified vitamin E and green tea microstructures were evaluated by the intestinal in situ single perfusion technique. Absorption rate, sub-acute oral toxicity and organ morphology in a rat model were examined. The intestinal in situ single perfusion technique and HPLC analysis were applied to investigate the absorption rate of selected materials by examining time-dependent changes in the serum levels of catechin and dl-α-tocopherol. The acute toxicity test and histopathological evaluation were applied to analyze the safety of microsized green tea and nanosized vitamin E in a rat model. Total serum dl-α-tocopherol levels significantly increased with nanosized vitamin E administration (P < 0.05). Rats treated to nanosized vitamin E until 90 min after administration showed significantly increased absorption rate of serum dl-α-tocopherol levels at each time point (10 min interval) (P < 0.001). Rats administered 2000 mg/kg of nanosized vitamin E and microsized green tea did not show signs of acute toxicity or death after 14 days of observation. In addition, macroscopic analysis showed that there were no changes in representative organ sections of rats following the oral administration of food-related nanoscale materials. We successfully demonstrated that using nanosized vitamin E increased absorption rate to a greater extent than normal food-related material, and these results occurs via safety analyses on food-related nanoscale materials for human consumption. These results could be useful for the design and development of novel nanoemulsified vitamin E and microsized green tea formulations that can overcome the problem of their bioavailability and improve their efficacy while still maintaining their essential therapeutic efficacies.

食品成分的吸收动力学，例如纳米乳化的维生素E和绿茶的微结构，通过肠道原位单次灌注技术进行了评估。检查大鼠模型的吸收率，亚急性口服毒性和器官形态。通过检查儿茶素和dl -α-生育酚的血清水平随时间的变化，采用肠道原位单次灌注技术和HPLC分析来研究所选材料的吸收率。应用急性毒性试验和组织病理学评估分析超小尺寸绿茶和纳米级维生素E在大鼠模型中的安全性。总血清dl纳米维生素E给药后，-α-生育酚水平显着增加（P  <0.05）。服用纳米维生素E直至给药90分钟后的大鼠在每个时间点（间隔10分钟）均显示血清dl -α-生育酚水平的吸收率显着提高（P <0.001）。观察到14天后，给予2000 mg / kg纳米维生素E和微型绿茶的大鼠没有显示出急性毒性或死亡的迹象。此外，宏观分析表明，口服食品相关纳米级材料后，大鼠的代表性器官切片没有变化。我们成功地证明，使用纳米级维生素E可以比普通食品相关材料更大程度地提高吸收率，这些结果是通过用于人类食用的食品相关纳米材料的安全性分析。这些结果可用于设计和开发新型纳米乳化的维生素E和微型绿茶配方，这些配方可以克服其生物利用度问题并提高其功效，同时仍保持其基本的治疗功效。