In this study, a new series of benzimidazole and bisbenzimidazole derivatives were prepared via the reaction of iminoester hydrochlorides and o-phenylenediamines and then screened for their lipase inhibition properties. Among the synthesized molecules, compounds 7a, 8a and 8c showed the best inhibitory effect against lipase enzyme with IC₅₀ values of 1.72 ± 0.12 µM, 1.92 ± 0.28 and 0.98 ± 0.07 µM, respectively. Moreover, molecular modeling studies were performed in order to understand to the inhibitory activity of the molecules. Binding poses of the studied compounds were determined at the target sites using induced fit docking (IFD) algorithms.

在这项研究中，通过亚氨基酯盐酸盐与邻苯二胺的反应制备了一系列新的苯并咪唑和双苯并咪唑衍生物，然后筛选了它们的脂肪酶抑制特性。在合成的分子中，化合物7a，8a和8c表现出对脂肪酶的最佳抑制作用，IC ₅₀值分别为1.72±0.12 µM，1.92±0.28和0.98±0.07 µM。此外，进行分子建模研究以了解分子的抑制活性。使用诱导拟合对接（IFD）算法在目标位点确定研究化合物的结合姿势。