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Synergistic peroxidase-like activity of CeO2-coated hollow Fe3O4 nanocomposites as an enzymatic mimic for low detection limit of glucose
Journal of the Taiwan Institute of Chemical Engineers ( IF 5.5 ) Pub Date : 2017-12-29 , DOI: 10.1016/j.jtice.2017.12.011
Fei Huang , Jianzhi Wang , Weimin Chen , Yinjia Wan , Xianming Wang , Ning Cai , Jie Liu , Faquan Yu

Fe3O4@CeO2 yolk–shell nanocomposites (NCs) were synthesized by growing CeO2 onto Fe3O4 hollow nanoparticles (NPs) as an artificial peroxidase for the purpose of elevated activity. The resultant NCs exhibited strong magnetic response with narrow size distribution and high surface area. Owing to the individual peroxidase-like catalytic activity of CeO2 onto Fe3O4 nanoparticles (NPs), Fe3O4@CeO2 NCs was investigated to achieve synergistic catalytic activity. As a result, the as-prepared Fe3O4@CeO2 NCs exhibited impressive peroxidase-like catalysis activity for 3, 3′, 5, 5′-tetramethylbenzidine (TMB). Moreover, comparing with natural enzymes, hollow Fe3O4 NPs and CeO2 NPs, the Fe3O4@CeO2 NCs displayed a lower Michaelis constant (Km) value or stronger affinity to H2O2 and TMB. A limit of detection of 8.5 µmol/L for H2O2 and 21 µmol/L for glucose was attained with acceptable stability and reproducibility. The activation mechanism was also studies using competitive radical tests and Electron spin resonance (ESR) analysis.



中文翻译:

CeO 2包覆的空心Fe 3 O 4纳米复合材料的过氧化物酶类协同活性作为低葡萄糖检测限的酶模拟物

Fe 3 O 4 @CeO 2卵黄壳纳米复合材料(NCs)是通过将CeO 2生长在Fe 3 O 4空心纳米颗粒(NPs)上作为人工过氧化物酶而合成的,目的是提高活性。所得的NC显示出强的磁响应,具有窄的尺寸分布和高的表面积。由于CeO 2对Fe 3 O 4纳米颗粒(NPs)具有类似过氧化物酶的催化活性,因此对Fe 3 O 4 @CeO 2 NCs进行了研究以达到协同催化活性。结果,制备的Fe 3 O 4@CeO 2 NCs对3,3',5,5'-四甲基联苯胺(TMB)表现出令人印象深刻的过氧化物酶样催化活性。此外,与天然酶,空心Fe 3 O 4 NP和CeO 2 NPs相比,Fe 3 O 4 @CeO 2 NCs的Michaelis常数(K m)值更低,或与H 2 O 2和TMB的亲和力更强。H 2 O 2的检出限为8.5 µmol / L葡萄糖达到21 µmol / L,并具有可接受的稳定性和可重复性。还使用竞争性自由基测试和电子自旋共振(ESR)分析研究了激活机制。

更新日期:2017-12-29
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