Abstract To disclosed the internal factors for the growth of mycelium and Lentinus edodes , Quercus Linn wood, which was biotransformed during the artificial cultivation of Lentinus edodes , were synergistically characterized by TGA/DTG, FT-IR and NMR. The results showed that the different ingredients of hemicellulose decreased during steam explosion and biodegradation of Lentinus edodes , however hemicellulose content continued to increased. FT-IR showed that the transmittance of the characteristic peaks in hemicellose gradually increased after decreased after steam explosion and biodegradation of Lentinus edodes . TGA/DTG curves that thermal stability and maximum thermal degradation rates of hemicelloses were contiguous after steam explosion and biodegradation of Lentinus edodes . Structural determination based on FT-IR and 1 H, and 2D-HSQC NMR analyses showed that the alkali-extractable hemicelluloses shared the structure composed of (1 → 4)-linked β- d -xylopyranosyl backbone with 4-O-methyl-R- d -glucuronic acid attached to O-2 of the xylose residues and l -arabinose attached to O-3 of the xylose residues. And it revealed that the extractable hemicelluloses retained original structure without cleaving chemical linkages. Furthermore, a small amount of other minor hemicelluloses (β-glucans) including xylans in the extractable hemicelluloses could be identified by NMR and other approaches.

中文翻译：

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香菇蒸汽预处理和生物降解过程中半纤维素结构的变化

摘要 为揭示香菇菌丝体和香菇生长的内在因素，对香菇人工栽培过程中生物转化的栎木进行了TGA/DTG、FT-IR和NMR协同表征。结果表明，香菇在蒸汽爆破和生物降解过程中，半纤维素的不同成分含量下降，而半纤维素含量继续增加。FT-IR表明，香菇经蒸汽爆破和生物降解后，半纤维素中特征峰的透光率逐渐降低后逐渐升高。香菇蒸汽爆破和生物降解后半纤维素的热稳定性和最大热降解率的TGA/DTG曲线是连续的。基于 FT-IR 和 1 H 的结构测定，和 2D-​​HSQC NMR 分析表明，可碱提取的半纤维素具有由 (1 → 4)-连接的 β-d-吡喃木糖主链和连接到 O-2 的 4-O-甲基-R-d-葡萄糖醛酸组成的结构。木糖残基和与木糖残基的 O-3 相连的 l-阿拉伯糖。并且它表明可提取的半纤维素保留了原始结构而没有裂解化学键。此外，可通过 NMR 和其他方法识别出少量其他次要半纤维素（β-葡聚糖），包括可提取半纤维素中的木聚糖。并且它表明可提取的半纤维素保留了原始结构而没有裂解化学键。此外，可通过 NMR 和其他方法识别出少量其他次要半纤维素（β-葡聚糖），包括可提取半纤维素中的木聚糖。并且它表明可提取的半纤维素保留了原始结构而没有裂解化学键。此外，可通过 NMR 和其他方法识别出少量其他次要半纤维素（β-葡聚糖），包括可提取半纤维素中的木聚糖。