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Integrating Metal-Enhanced Fluorescence and Surface Acoustic Waves for Sensitive and Rapid Quantification of Cancer Biomarkers from Real Matrices
ACS Sensors ( IF 8.2 ) Pub Date : 2018-01-16 00:00:00 , DOI: 10.1021/acssensors.7b00876 Jun Liu 1 , Shuangming Li 1 , Venkat R. Bhethanabotla 1
ACS Sensors ( IF 8.2 ) Pub Date : 2018-01-16 00:00:00 , DOI: 10.1021/acssensors.7b00876 Jun Liu 1 , Shuangming Li 1 , Venkat R. Bhethanabotla 1
Affiliation
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Metal-enhanced fluorescence (MEF) is utilized to lower the detection limit of carcinoembryonic antigen (CEA), a prognostic biomarker for colorectal cancer among others, in immunofluorescence assays. In addition, Rayleigh surface acoustic waves (SAWs) were utilized to remove nonspecifically bound proteins, improve mixing, and reduce incubation times. Fluorescence intensity was plasmonically enhanced by incubating silver nanocubes (AgNCs) of 50 nm edge-length on a SAW device. This increased sensor sensitivity by a factor of 6 and lowered the limit of detection to below 1 ng/mL in fluorescence detection of the antigen. Surface density of the AgNCs was optimized to produce the largest MEF, which increased the signal intensity by an order of magnitude. Acoustic streaming induced by Rayleigh SAWs was found to decrease antibody/antigen incubation times to 1/6th of the values without such micromixing, and to increase the fluorescence signal strength. Overall, the demonstrated results allow for construction of a sensor capable of detecting CEA rapidly in clinically relevant concentrations. Variables relevant for optimizing this sensor performance were identified, which will enable even better performance in immunofluorescence assays.
中文翻译：
集成金属增强的荧光和表面声波，用于灵敏，快速地定量来自真实基质的癌症生物标志物
在免疫荧光分析中，金属增强荧光（MEF）用于降低癌胚抗原（CEA）的检出限，CEA是结肠直肠癌的一种预后生物标志物。此外，瑞利表面声波（SAW）用于去除非特异性结合的蛋白质，改善混合并减少孵育时间。通过在SAW器件上孵育50 nm边长的银纳米立方体（AgNC），可增强等离子体强度的荧光强度。在抗原的荧光检测中，传感器的灵敏度提高了6倍，并将检测限降低到1 ng / mL以下。优化了AgNC的表面密度，以产生最大的MEF，从而将信号强度提高了一个数量级。发现由瑞利声表面波诱导的声流可将抗体/抗原的孵育时间减少到不进行此类微混合的值的1/6，并增加了荧光信号强度。总的来说，证明的结果允许构建能够在临床相关浓度下快速检测CEA的传感器。确定了与优化此传感器性能相关的变量，这将使免疫荧光测定具有更好的性能。
更新日期：2018-01-16
中文翻译：
集成金属增强的荧光和表面声波，用于灵敏，快速地定量来自真实基质的癌症生物标志物
在免疫荧光分析中，金属增强荧光（MEF）用于降低癌胚抗原（CEA）的检出限，CEA是结肠直肠癌的一种预后生物标志物。此外，瑞利表面声波（SAW）用于去除非特异性结合的蛋白质，改善混合并减少孵育时间。通过在SAW器件上孵育50 nm边长的银纳米立方体（AgNC），可增强等离子体强度的荧光强度。在抗原的荧光检测中，传感器的灵敏度提高了6倍，并将检测限降低到1 ng / mL以下。优化了AgNC的表面密度，以产生最大的MEF，从而将信号强度提高了一个数量级。发现由瑞利声表面波诱导的声流可将抗体/抗原的孵育时间减少到不进行此类微混合的值的1/6，并增加了荧光信号强度。总的来说，证明的结果允许构建能够在临床相关浓度下快速检测CEA的传感器。确定了与优化此传感器性能相关的变量，这将使免疫荧光测定具有更好的性能。
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