Microbial production of fatty acid-derived hydrocarbons offers a great opportunity to sustainably supply biofuels and oleochemicals. One challenge is to achieve a high production rate. Besides, low efficiency in secretion will cause high separation costs, and it is therefore desirable to have product secretion. Here, we engineered the budding yeast Saccharomyces cerevisiae to produce and secrete 1-alkenes by manipulation of the fatty acid metabolism, enzyme selection, engineering the electron transfer system and expressing a transporter. Furthermore, we implemented a dynamic regulation strategy to control the expression of membrane enzyme and transporter, which improved 1-alkene production and cell growth by relieving the possible toxicity of overexpressed membrane proteins. With these efforts, the engineered yeast cell factory produced 35.3 mg/L 1-alkenes with more than 80% being secreted. This represents a 10-fold improvement compared with earlier reported hydrocarbon production by S. cerevisiae.

中文翻译：

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动态调节酵母中的1-烯生物合成和分泌工程

微生物衍生的脂肪酸衍生的碳氢化合物为可持续地供应生物燃料和油脂化学品提供了巨大的机会。一个挑战是实现高生产率。此外，分泌效率低将导致高分离成本，因此期望具有产物分泌。在这里，我们设计了出芽酵母酿酒酵母通过控制脂肪酸代谢，酶的选择，设计电子转移系统并表达转运蛋白来生产和分泌1-烯烃。此外，我们实施了动态调节策略来控制膜酶和转运蛋白的表达，从而通过减轻过表达的膜蛋白的毒性来改善1-烯烃的生产和细胞生长。通过这些努力，工程酵母细胞工厂生产了35.3 mg / L的1-烯烃，其中80％以上被分泌出来。与较早报道的酿酒酵母的烃生产相比，这代表了十倍的改进。