Hydrogen sulfide (H₂S) is now recognized as an important gaseous transmitter that is involved in a variety of biological processes. Here, we report the design and synthesis of a luminescent lanthanide biosensor for H₂S, LP2-Cu(II)-Ln(III), a heterobinuclear metal complex that uses Cu(II) decomplexation to control millisecond-scale-lifetime-Tb(III)- or Eu(III)-emission intensity. LP2-Cu(II)-Ln(III) responded rapidly, selectively, and with high sensitivity to aqueous H₂S. The probe’s potential for biological applications was verified by measuring the H₂S generated by the slow-releasing chemical-sulfide-donor GYY4147, by cystathionine γ-lyase (CSE), and by Na₂S-stimulated HeLa cells.

中文翻译：

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酶促产生的硫化氢的时间门控发光检测：基于镧系元素的探针的设计，合成和应用

如今，硫化氢（H ₂ S）被认为是一种重要的气体传输剂，它参与了多种生物过程。在这里，我们报告为H ₂ S，LP2-Cu（II）-Ln（III）（一种使用Cu（II）分解来控制毫秒级寿命-Tb的异双核金属配合物）的发光镧系元素生物传感器的设计和合成（III）或Eu（III）的发射强度。LP2-Cu（II）-Ln（III）对H ₂ S水溶液具有快速，选择性和高灵敏度的响应。通过测量缓慢释放的化学硫化物产生的H ₂ S可以验证该探针在生物学上的潜力。胱硫醚γ-裂合酶（CSE）和Na ₂ S刺激的HeLa细胞作为供体GYY4147 。