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Quantification of Nucleic Acid Concentration in the Nanoparticle or Polymer Conjugates Using Circular Dichroism Spectroscopy
Analytical Chemistry ( IF 6.7 ) Pub Date : 2018-01-12 00:00:00 , DOI: 10.1021/acs.analchem.7b04621 Zhili Peng 1, 2 , Jiaojiao Li 3 , Shanghao Li 2, 4 , Joel Pardo 2 , Yiqun Zhou 2 , Abdulrahman O. Al-Youbi 5 , Abdulaziz S. Bashammakh 5 , Mohammad S. El-Shahawi 5 , Roger M. Leblanc 2
Analytical Chemistry ( IF 6.7 ) Pub Date : 2018-01-12 00:00:00 , DOI: 10.1021/acs.analchem.7b04621 Zhili Peng 1, 2 , Jiaojiao Li 3 , Shanghao Li 2, 4 , Joel Pardo 2 , Yiqun Zhou 2 , Abdulrahman O. Al-Youbi 5 , Abdulaziz S. Bashammakh 5 , Mohammad S. El-Shahawi 5 , Roger M. Leblanc 2
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The interface of nucleic acids and nanomaterials is among the most promising fields in recent years. Considerable efforts have been devoted to the development of novel systems based on the two components for various promising applications such as sensing, bioimaging, drug delivery, and theranostics. However, the determination of nucleic acid concentration in these systems remains as a challenge due to the interference of nanoparticles. To this end, we developed a simple, yet reliable, method to quantify the nucleic acid concentration in their nanoparticle or polymer conjugates based on circular dichroism (CD) spectroscopy. In this paper, three nucleic acids, namely, DNA sodium salt from calf thymus (NaDNA), DNA from herring sperm (hsDNA), and ribonucleic acid from torula yeast (tyRNA), were noncovalently conjugated to three nanoparticles. The concentrations of the three nucleic acids in their nanoparticle conjugates were successfully determined on the basis of CD spectra calibration curves.
中文翻译：
使用圆二色光谱法定量分析纳米粒子或聚合物共轭物中的核酸浓度
核酸和纳米材料的界面是近年来最有前途的领域之一。基于这两个组件的新型系统的开发已经投入了大量的精力，用于各种有前途的应用，例如传感，生物成像，药物输送和治疗学。然而，由于纳米颗粒的干扰，在这些系统中确定核酸浓度仍然是一个挑战。为此，我们开发了一种简单而可靠的方法，可基于圆二色性（CD）光谱法定量其纳米颗粒或聚合物共轭物中的核酸浓度。在本文中，三种核酸，即小牛胸腺的DNA钠盐（NaDNA），鲱鱼的精子的DNA盐（hsDNA）和to属酵母的核糖核酸（tyRNA）非共价结合到三个纳米颗粒上。
更新日期：2018-01-12
中文翻译：
使用圆二色光谱法定量分析纳米粒子或聚合物共轭物中的核酸浓度
核酸和纳米材料的界面是近年来最有前途的领域之一。基于这两个组件的新型系统的开发已经投入了大量的精力，用于各种有前途的应用，例如传感，生物成像，药物输送和治疗学。然而，由于纳米颗粒的干扰，在这些系统中确定核酸浓度仍然是一个挑战。为此，我们开发了一种简单而可靠的方法，可基于圆二色性（CD）光谱法定量其纳米颗粒或聚合物共轭物中的核酸浓度。在本文中，三种核酸，即小牛胸腺的DNA钠盐（NaDNA），鲱鱼的精子的DNA盐（hsDNA）和to属酵母的核糖核酸（tyRNA）非共价结合到三个纳米颗粒上。
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