General and Facile Coating of Single Cells via Mild Reduction,Journal of the American Chemical Society

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General and Facile Coating of Single Cells via Mild Reduction
Journal of the American Chemical Society ( IF 14.4 ) Pub Date : 2018-01-05 , DOI: 10.1021/jacs.7b08440
Hyunbum Kim ₁ , Kwangsoo Shin _{1,

2} , Ok Kyu Park ₂ , Daheui Choi ₃ , Hwan D. Kim ₁ , Seungmin Baik _{1,

2} , Soo Hong Lee _{1,

2} , Seung-Hae Kwon ₄ , Kevin J. Yarema ₅ , Jinkee Hong ₃ , Taeghwan Hyeon _{1,

2} , Nathaniel S. Hwang ₁

Affiliation

Cell surface modification has been extensively studied to enhance the efficacy of cell therapy. Still, general accessibility and versatility are remaining challenges to meet the increasing demand for cell-based therapy. Herein, we present a facile and universal cell surface modification method that involves mild reduction of disulfide bonds in cell membrane protein to thiol groups. The reduced cells are successfully coated with biomolecules, polymers, and nanoparticles for an assortment of applications, including rapid cell assembly, in vivo cell monitoring, and localized cell-based drug delivery. No adverse effect on cellular morphology, viability, proliferation, and metabolism is observed. Furthermore, simultaneous coating with polyethylene glycol and dexamethasone-loaded nanoparticles facilitates enhanced cellular activities in mice, overcoming immune rejection.

中文翻译：

通过温和还原对单个细胞进行一般和简便的涂层

细胞表面修饰已被广泛研究以提高细胞治疗的功效。尽管如此，一般的可访问性和多功能性仍然是满足对基于细胞的治疗日益增长的需求的挑战。在此，我们提出了一种简便且通用的细胞表面修饰方法，该方法涉及将细胞膜蛋白中的二硫键温和还原为硫醇基团。还原的细胞成功地被生物分子、聚合物和纳米粒子包被，用于各种应用，包括快速细胞组装、体内细胞监测和基于细胞的局部药物递送。未观察到对细胞形态、活力、增殖和代谢的不利影响。此外，同时包覆聚乙二醇和地塞米松纳米颗粒可促进小鼠细胞活动的增强，

更新日期：2018-01-05

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