Functional analysis of lymphocytes is important for development of vaccines and diagnosis/treatment of various immune-related diseases. In this review, we describe multifunctional microwell arrays that enable functional analysis of lymphocytes at the single cell level. We first discuss key parameters for microwell array design. Then, we describe how different types of multifunctional microwell arrays were developed for various applications, including live cell imaging of lymphocyte activation, proliferation, and differentiation, and analyses of effector functions such as cytokine secretion and target cell lysis. Incorporation of novel surface chemistries and functional materials into microwell arrays for enhancing sensing capabilities will widen applications of this technology. Multifunctional microwell arrays will be a powerful tool for the development of novel therapeutics against immune-related diseases, in particular, for cancer immunotherapy.

中文翻译：

---

用于淋巴细胞单细胞水平功能分析的多功能微孔阵列

淋巴细胞的功能分析对于疫苗的开发和各种免疫相关疾病的诊断/治疗很重要。在这篇综述中，我们描述了多功能微孔阵列，能够在单细胞水平上对淋巴细胞进行功能分析。我们首先讨论微孔阵列设计的关键参数。然后，我们描述了如何为各种应用开发不同类型的多功能微孔阵列，包括淋巴细胞激活，增殖和分化的活细胞成像，以及效应子功能（如细胞因子分泌和靶细胞裂解）的分析。将新的表面化学和功能材料结合到微孔阵列中以增强感测能力将扩大该技术的应用范围。