The elimination of disease-causing microbes from the food supply is a primary goal and this review deals with the overall techniques available for detection of food-borne pathogens. Now-a-days conventional methods are replaced by advanced methods like Biosensors, Nucleic Acid-based Tests (NAT), and different PCR-based techniques used in molecular biology to identify specific pathogens. Bacillus cereus, Staphylococcus aureus, Proteus vulgaris, Escherichia coli, Campylobacter, Listeria monocytogenes, Salmonella spp., Aspergillus spp., Fusarium spp., Penicillium spp., and pathogens are detected in contaminated food items that cause always diseases in human in any one or the other way. Identification of food-borne pathogens in a short period of time is still a challenge to the scientific field in general and food technology in particular. The low level of food contamination by major pathogens requires specific sensitive detection platforms and the present area of hot research looking forward to new nanomolecular techniques for nanomaterials, make them suitable for the development of assays with high sensitivity, response time, and portability. With the sound of these, we attempt to highlight a comprehensive overview about food-borne pathogen detection by rapid, sensitive, accurate, and cost affordable in situ analytical methods from conventional methods to recent molecular approaches for advanced food and microbiology research.

中文翻译：

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用于检测食源性病原体的先进分子诊断技术：当前的应用和未来的挑战

消除食品供应中的致病微生物是一个主要目标，本综述涉及可用于检测食源性病原体的整体技术。如今，传统方法已被生物传感器，基于核酸的测试（NAT）和分子生物学中用于识别特定病原体的不同基于PCR的技术等先进方法所取代。蜡状芽孢杆菌，金黄色葡萄球菌，变形杆菌，大肠杆菌，弯曲杆菌，单核细胞增生李斯特菌，沙门氏菌，曲霉，镰刀菌，青霉菌和病原体均在被污染的食物中被发现，这些食物总是导致人类的疾病或其他方式。在短时间内鉴定食源性病原体仍然是对整个科学领域特别是食品技术的挑战。主要病原体对食品的污染程度低，需要特定的灵敏检测平台，并且当前的热门研究领域期待纳米材料的新型纳米分子技术，使其适合开发具有高灵敏度，响应时间和便携性的测定方法。有了这些声音，我们尝试着重介绍通过快速，灵敏，准确且价格合理的原位分析方法对食源性病原体进行检测的全面概述，从传统方法到用于高级食品和微生物学研究的最新分子方法。使它们适合于具有高灵敏度，响应时间和便携性的测定方法的开发。有了这些声音，我们尝试着重介绍通过快速，灵敏，准确且价格合理的原位分析方法对食源性病原体进行检测的全面概述，从传统方法到用于高级食品和微生物学研究的最新分子方法。使它们适合于具有高灵敏度，响应时间和便携性的测定方法的开发。有了这些声音，我们尝试着重介绍通过快速，灵敏，准确且价格合理的原位分析方法对食源性病原体进行检测的全面概述，从传统方法到用于高级食品和微生物学研究的最新分子方法。