In this work, a spore-based biosensor is evaluated to monitor the microbicidal efficacy of sterilization processes applying gaseous hydrogen peroxide (H₂O₂). The sensor is based on interdigitated electrode structures (IDEs) that have been fabricated by means of thin-film technologies. Impedimetric measurements are applied to study the effect of sterilization process on spores of Bacillus atrophaeus. This resilient microorganism is commonly used in industry to proof the sterilization efficiency. The sensor measurements are accompanied by conventional microbiological challenge tests, as well as morphological characterizations with scanning electron microscopy (SEM) and transmission electron microscopy (TEM). The sensor measurements are correlated with the microbiological test routines. In both methods, namely the sensor-based and microbiological one, a tailing effect has been observed. The results are evaluated and discussed in a three-dimensional calibration plot demonstrating the sensor's suitability to enable a rapid process decision in terms of a successfully performed sterilization.

在这项工作中，对基于孢子的生物传感器进行了评估，以监测使用气态过氧化氢（H ₂ O ₂）的灭菌过程的杀菌效力。该传感器基于通过薄膜技术制造的叉指式电极结构（IDE）。阻抗测量用于研究灭菌过程对萎缩芽孢杆菌孢子的影响。这种有回弹力的微生物通常在工业上用于证明灭菌效率。传感器的测量伴随着常规的微生物挑战性测试以及扫描电子显微镜（SEM）和透射电子显微镜（TEM）的形态表征。传感器的测量值与微生物检测程序相关。在两种方法中，即基于传感器的方法和微生物方法，均已观察到拖尾效应。在三维校准图中对结果进行评估和讨论，展示了传感器的适用性，从而能够根据成功执行的灭菌过程快速做出决策。