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Antioxidative and antibacterial activities of aqueous ethanol extracts of berries, leaves, and branches of berry plants
Food Research International ( IF 7.0 ) Pub Date : 2017-12-27 , DOI: 10.1016/j.foodres.2017.12.071
Ye Tian , Anna Puganen , Hanna-Leena Alakomi , Aleksi Uusitupa , Maria Saarela , Baoru Yang

Phenolic compounds were extracted with food grade solvent of acidified aqueous ethanol from leaves, berries, berry press cakes, and branches of Finnish berry plants and analyzed with HPLC-DAD, UPLC-DAD-ESI-MS and NMR. In addition, press cakes from two berry species and branches from one species were also extracted and analyzed with the same methods. The antioxidant activities of the extracts were evaluated using Folin-Ciocalteau, oxygen radical absorbance capacity (ORAC), DPPH free radical scavenging, and total radical trapping antioxidant parameter (TRAP) assays. The antibacterial activities were investigated against various Gram-negative and Gram-positive foodborne pathogens. The leaf extracts showed higher antioxidative activities (3–20 fold in ORAC assay, 10–20 fold in TRAP) than the berry extracts, in association with the higher contents of phenolic compounds in the leaf extracts; Strongest anti-bacterial effects was observed in the leaf extracts of lingonberry (Vaccinium vitis-idaea), sea buckthorn (Hippophaë rhamnoides ssp. rhamnoides) and saskatoon (Amelanchier alnifolia) on Staphylococcus aureus, Listeria monocytogenes, and Bacillus cereus. However, the antibacterial efficacy varied with bacterial species and strains. The Folin-Ciocalteu, ORAC, and TRAP values was strongly correlated with the total content of flavonoids with less association shown with the content of total phenolics and flavonol glycosides. The results suggest a major contribution of pranthocyanidins and flavan‐3-ols to the antioxidative activities of the extracts. The growth inhibition on Staphylococcus aureus and Bacillus cereus was clearly associated with the content of total phenolics and ellagitannins.



中文翻译:

浆果植物的浆果,叶和树枝的乙醇水溶液提取物的抗氧化和抗菌活性

用酸化乙醇的食品级溶剂从芬兰浆果植物的叶子,果实,浆果压榨蛋糕和分支中提取酚类化合物,并用HPLC-DAD,UPLC-DAD-ESI-MS和NMR进行分析。此外,还提取和分析了两种浆果的压滤饼和一种浆果的压条。使用Folin-Ciocalteau,氧自由基吸收能力(ORAC),DPPH自由基清除和总自由基捕获抗氧化剂参数(TRAP)分析评估提取物的抗氧化活性。研究了对各种革兰氏阴性和革兰氏阳性食源性病原菌的抗菌活性。叶提取物显示出比浆果提取物更高的抗氧化活性(在ORAC分析中为3–20倍,在TRAP分析中为10–20倍),与叶提取物中酚类化合物含量较高有关;在越橘的叶提取物中观察到最强的抗菌作用(越橘葡萄- idaea),沙棘(沙棘亚种沙棘)和萨斯卡通(桤alnifolia上)金黄色葡萄球菌李斯特菌蜡状芽孢杆菌。但是,抗菌效果随细菌种类和菌株的不同而不同。Folin-Ciocalteu,ORAC和TRAP值与黄酮类化合物的总含量密切相关,而总酚类和黄酮醇苷的含量则显示较少的相关性。结果表明,花青素和黄烷-3-醇对提取物的抗氧化活性有重要贡献。对金黄色葡萄球菌的生长抑制蜡样芽孢杆菌显然与总酚和鞣花单宁的含量有关。

更新日期:2017-12-27
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