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Pepper mild mottle virus as a process indicator at drinking water treatment plants employing coagulation-sedimentation, rapid sand filtration, ozonation, and biological activated carbon treatments in Japan
Water Research ( IF 11.4 ) Pub Date : 2017-12-27 , DOI: 10.1016/j.watres.2017.12.068
Ryuichi Kato , Tatsuya Asami , Etsuko Utagawa , Hiroaki Furumai , Hiroyuki Katayama

To assess the potential of pepper mild mottle virus (PMMoV) as a viral process indicator, its reduction through coagulation–sedimentation (CS) and rapid sand filtration (RSF) were compared with those of Escherichia coli, previously used viral indicators, and norovirus genotype II (NoV GII; enteric virus reference pathogen) in a bench-scale experiment. PMMoV log10 reductions in CS (1.96 ± 0.30) and RSF (0.26 ± 0.38) were similar to those of NoV GII (1.86 ± 0.61 and 0.28 ± 0.46). PMMoV, the most abundant viruses in the raw water, was also determined during CS, RSF, and advanced treatment processes at two full-scale drinking water treatment plants under strict turbidity management over a 13-month period. PMMoV was concentrated from large-volume water samples (10–614 L) and quantified by Taqman-based quantitative polymerase chain reaction. The PMMoV log10 reduction in CS (2.38 ± 0.74, n = 13 and 2.63 ± 0.76, n = 10 each for Plant A and B) and in ozonation (1.91 ± 1.18, n = 5, Plant A) greatly contributed to the overall log10 reduction. Our results suggest that PMMoV can act as a useful treatment process indicator of enteric viruses and can be used to monitor the log10 reduction of individual treatment processes at drinking water treatment plants due to its high and consistent copy numbers in source water.



中文翻译:

辣椒轻度斑驳病毒在日本采用混凝沉淀,快速砂滤,臭氧化和生物活性炭处理的饮用水处理厂中作为过程指标

为了评估胡椒轻斑驳病毒(PMMoV)作为病毒过程指标的潜力,将其通过凝结沉淀(CS)和快速砂滤(RSF)的减少与大肠杆菌,以前使用的病毒指标和诺如病毒基因型进行了比较。 II(NoV GII;肠病毒参考病原体)进行基准试验。PMMoV日志10CS(1.96±0.30)和RSF(0.26±0.38)的降低与NoV GII的降低(1.86±0.61和0.28±0.46)相似。PMMoV是原水中最丰富的病毒,也是在CS,RSF和高级处理过程中,在严格的浊度管理下经过13个月的时间,在两家大型饮用水处理厂中确定的。PMMoV从大量水样(10–614 L)中浓缩,并通过基于Taqman的定量聚合酶链反应进行定量。CS和PMMoV log 10的减少量(植物A和B分别为2.38±0.74,n = 13和2.63±0.76,n = 10)和臭氧化作用(1.91±1.18,n = 5,植物A)的降低很大日志10减少。我们的结果表明,PMMoV可以作为肠道病毒治疗过程的有用指示剂,并且由于其在源水中的拷贝数较高且一致,因此可用于监测饮用水处理厂中各个处理过程的log 10减少。

更新日期:2017-12-27
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