A biomolecule‐recognition gating membrane, which introduces thermosensitive graft polymer including molecular recognition receptor into porous membrane substrate, can close its pores by recognizing target biomolecule. The present study reports strategies for improving both versatility and sensitivity of the gating membrane. First, the membrane is fabricated by introducing the receptor via a selectively reactive click reaction improving the versatility. Second, the sensitivity of the membrane is enhanced via an active delivering method of the target molecules into the pores. In the method, the tiny signal of the target biomolecule is amplified as obvious pressure change. Furthermore, this offers 15 times higher sensitivity compared to the previously reported passive delivering method (membrane immersion to sample solution) with significantly shorter recognition time. The improvement will aid in applying the gating membrane to membrane sensors in medical fields.

中文翻译：

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生物分子识别门控膜在小孔空间内控制目标分子识别

生物分子识别门控膜将包括分子识别受体的热敏接枝聚合物引入多孔膜基质中，可以通过识别目标生物分子来封闭其孔。本研究报告了改善通用性和门控膜灵敏度的策略。首先，通过经由选择性反应性点击反应引入受体来改善通用性来制造膜。其次，通过将靶分子主动递送到孔中的方法增强了膜的敏感性。该方法将目标生物分子的微小信号放大为明显的压力变化。此外，与以前报道的被动传递方法（将膜浸入样品溶液中）相比，这提供了15倍的灵敏度，并且识别时间大大缩短。该改进将有助于将门控膜应用于医疗领域的膜传感器。