Herein a label-free immunosensor based on electrolyte-gated organic field-effect transistor (EGOFET) was developed for the detection of procalcitonin (PCT), a sepsis marker. Antibodies specific to PCT were immobilized on the poly-3-hexylthiophene (P3HT) organic semiconductor surface through direct physical adsorption followed by a post-treatment with bovine serum albumin (BSA) which served as the blocking agent to prevent non-specific adsorption. Antibodies together with BSA (forming the whole biorecognition layer) served to selectively capture the procalcitonin target analyte. The entire immunosensor fabrication process was fast, requiring overall 45 min to be completed before analyte sensing. The EGOFET immunosensor showed excellent electrical properties, comparable to those of bare P3HT based EGOFET confirming reliable biosensing with bio-functional EGOFET immunosensor. The detection limit of the immunosensor was as low as 2.2 pM and within a range of clinical relevance. The relative standard deviation of the individual calibration data points, measured on immunosensors fabricated on different chips (reproducibility error) was below 7%. The developed immunosensor showed high selectivity to the PCT analyte which was evident through control experiments. This report of PCT detection is first of its kind among the electronic sensors based on EGOFETs. The developed sensor is versatile and compatible with low-cost fabrication techniques.

在本文中，开发了基于电解质门控有机场效应晶体管（EGOFET）的无标记免疫传感器，用于检测脓毒症标志物降钙素（PCT）。PCT特异抗体通过直接物理吸附固定在聚3-己基噻吩（P3HT）有机半导体表面上，然后用牛血清白蛋白（BSA）作后处理，牛血清白蛋白用作阻止非特异性吸附的封闭剂。抗体与BSA（形成整个生物识别层）一起用于选择性捕获降钙素原目标分析物。整个免疫传感器的制造过程非常快，需要整个45分钟才能完成分析物感测。EGOFET免疫传感器显示出优异的电性能，与基于裸露的基于P3HT的EGOFET相比，证实了具有生物功能的EGOFET免疫传感器的可靠生物传感。免疫传感器的检出限低至2.2 pM，且在临床相关范围内。在不同芯片上制造的免疫传感器上测得的各个校准数据点的相对标准偏差（再现性误差）低于7％。研发的免疫传感器对PCT分析物显示出很高的选择性，这通过对照实验可以明显看出。这份有关PCT检测的报告是基于EGOFET的电子传感器中的首例。研发的传感器用途广泛，并与低成本制造技术兼容。在不同芯片上制造的免疫传感器上测得的各个校准数据点的相对标准偏差（再现性误差）低于7％。研发的免疫传感器对PCT分析物显示出很高的选择性，这通过对照实验可以明显看出。这份有关PCT检测的报告是基于EGOFET的电子传感器中的首例。研发的传感器用途广泛，并与低成本制造技术兼容。在不同芯片上制造的免疫传感器上测得的各个校准数据点的相对标准偏差（再现性误差）低于7％。研发的免疫传感器对PCT分析物显示出很高的选择性，这通过对照实验可以明显看出。这份有关PCT检测的报告是基于EGOFET的电子传感器中的首例。开发的传感器用途广泛，可与低成本制造技术兼容。