A novel diphenylalaninamid (FFA) based peptide nanoparticles (PNPs) modified pencil graphite electrodes (PGEs) for construction of electrochemical cytosensor was demonstrated for the first time in this study. Scanning electron microscopy (SEM) and transmission electron microscopy (TEM) images revealed the spherical nanostructure of the synthesized FFA based PNPs while attenuated total reflectance-fourier transform infrared (ATR-FTIR) spectra provided information about the structure and conformation of proteins in their structure. Self-assembly of PNPs on PGE surface and adhesion of DLD-1 cancer cells on this surface was also characterized by electrochemical measurements. PNP/PGEs acted as a sensitive platform for simple and rapid quantification of low concentration of DLD-1 cancer cells in early diagnosis using the electrochemical impedance method (EIS). The offered cytosensor demonstrated outstanding performance for the detection of DLD-1 cells by the EIS method. The impedance of electronic transduction was associated with the amount of the immobilized cells ranging from 2 × 10² to 2.0 × 10⁵ cells mL⁻¹ with a limit of detection of 100 cells mL⁻¹. The efficient performance of the cytosensor was attributed to the well-defined nanostructure and biocompability of PNPs on the substrate.

这项研究中首次展示了一种新型的基于二苯丙氨酸酰胺（FFA）的肽纳米颗粒（PNPs）修饰的铅笔状石墨电极（PGEs）。扫描电子显微镜（SEM）和透射电子显微镜（TEM）图像显示了合成的基于FFA的PNP的球形纳米结构，而衰减的全反射-傅立叶变换红外（ATR-FTIR）光谱提供了有关蛋白质结构及其结构构象的信息。还通过电化学测量表征了PGE在PGE表面上的自组装和DLD-1癌细胞在该表面上的粘附。PNP / PGEs用作使用电化学阻抗法（EIS）在早期诊断中对低浓度DLD-1癌细胞进行简单，快速定量的敏感平台。所提供的细胞传感器展示了通过EIS方法检测DLD-1细胞的出色性能。电子转导的阻抗与固定细胞的数量相关，范围为2×10²至2.0×10 ^5个细胞mL ^-1，检测限为100个细胞mL ^-1。细胞传感器的有效性能归因于底物上PNP的明确定义的纳米结构和生物相容性。